PMID- 16640906
OWN - NLM
STAT- MEDLINE
DCOM- 20090420
LR  - 20131121
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 40
IP  - 2
DP  - 2006 Mar
TI  - [Enhancement effect of polychlorinated biphenyl on benzo (a) pyrene-induced DNA 
      damage in HepG2 cells].
PG  - 97-100
AB  - OBJECTIVE: To study the effect of polychlorinated biphenyl, Aroclor1254 on benzo 
      (a) pyrene [B (a) P]-induced DNA damage in HepG2 cells. METHODS: HepG2 cells were 
      pretreated with Aroclor1254 (11.5, 23 and 46 micromol/L) for 24 hours and then 
      exposed to B (a) P (50 micromol/L). DMSO (10 ml/L) was used as solvent control. 
      Single cell gel electrophoresis (SCGE) and high-performance liquid 
      chromatography-electrochemical detection (HPLC-EC) assays were applied to detect 
      DNA single-strand breaks and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in HepG2 cells, 
      respectively. RESULTS: Average Oliver tail moment (OTM) and 8-OHdG level in HepG2 
      cells were significantly increased in B (a) P treated group (1.66 +/- 0.21), 
      (23.31 +/- 6.02) 8-OHdG/10(6)dG than that in solvent control (0.79 +/- 0.15), 
      (12.31 +/- 3.24) 8-OHdG/10(6)dG, respectively. In Aroclor 1254 treated group 
      (11.5, 23.0, 46.0 micromol/L), average OTM were 0.88 +/- 0.20, 1.01 +/- 0.15 and 
      1.10 +/- 0.16, and 8-OHdG levels were (19.57 +/- 7.57), (22.80 +/- 9.16) and 
      (31.74 +/- 9.25) 8-OHdG/10(6)dG, respectively. A concentration of 46 micromol/L 
      Aroclor1254 caused a significant increase of 8-OHdG level as compared with the 
      solvent control. After pretreatment of HepG2 cells with Aroclor1254 (11.5, 23.0 
      and 46.0 micromol/L), B (a) P induced more DNA strand breaks (OTM: 2.14 +/- 0.22, 
      2.43 +/- 0.32 and 2.71 +/- 0.31) and 8-OHdG [(32.50 +/- 3.81), (49.23 +/- 16.66) 
      and (60.36 +/- 18.04) 8-OHdG/10(6)dG] in HepG2 cells than B (a) P alone. 
      CONCLUSION: Aroclor1254 might enhance B (a) P-induced DNA damage in HepG2 cells, 
      which should imply a synergistic effect of Aroclor1254 on the genotoxicity of B 
      (a) P.
FAU - Zou, Ya-ling
AU  - Zou YL
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan 
      430030, China.
FAU - Lai, Rui-ping
AU  - Lai RP
FAU - Zhou, Li-hong
AU  - Zhou LH
FAU - Li, Xiao-yan
AU  - Li XY
FAU - Lu, Wen-qing
AU  - Lu WQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Benzo(a)pyrene/*toxicity
MH  - Cell Line, Tumor
MH  - DNA Damage/*drug effects
MH  - Drug Synergism
MH  - Humans
MH  - Polychlorinated Biphenyls/*toxicity
EDAT- 2006/04/28 09:00
MHDA- 2009/04/21 09:00
CRDT- 2006/04/28 09:00
PHST- 2006/04/28 09:00 [pubmed]
PHST- 2009/04/21 09:00 [medline]
PHST- 2006/04/28 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Mar;40(2):97-100.