PMID- 16641917
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 69
IP  - 11
DP  - 2006 Jun
TI  - Oxidative stress during peritoneal dialysis: implications in functional and 
      structural changes in the membrane.
PG  - 2022-8
AB  - Progressive peritoneal fibrosis, membrane hyperpermeability, and ultrafiltration 
      failure have been observed in patients on long-term peritoneal dialysis (PD). The 
      present study tested the hypothesis that reactive oxygen species (ROS) generated 
      by conventional PD solution (PDS) mediate functional and structural alterations 
      of peritoneal membrane in vivo. Sprague-Dawley rats were randomized to control, 
      PDS, PDS with an antioxidant, and PDS with an angiotensin II (Ang II) receptor 
      blocker. Commercial PDS containing 3.86% glucose (20-30 ml) with or without 
      N-acetylcystein (NAC) 10 mM or losartan 5 mg/kg was administered 
      intraperitoneally twice a day for 12 weeks. Control rats received sham injection. 
      Rats treated with PDS had significantly lower drain volume and D(4)/D(0) glucose, 
      but higher D(4)/P(4) creatinine and increased membrane thickness and endothelial 
      NOS (eNOS) expression compared to control rats. Omental transforming growth 
      factor (TGF)-beta1, vascular endothelial growth factor (VEGF), collagen I, and 
      heat-shock protein (hsp) 47 expression and lipid peroxide levels and dialysate 
      VEGF and Ang II concentrations were significantly increased in rats treated with 
      PDS compared to control. All of these changes were prevented by both NAC and 
      losartan. In conclusion, the present study demonstrates that ROS generated by 
      conventional PDS are, in large part, responsible for peritoneal fibrosis and 
      membrane hyperpermeability. We suggest that antioxidants or Ang II receptor 
      blockers may allow better preservation of the structural and functional integrity 
      of the peritoneal membrane during long-term PD.
FAU - Noh, H
AU  - Noh H
AD  - Hyonam Kidney Laboratory, Soon Chun Hyang University, Seoul, Korea.
FAU - Kim, J S
AU  - Kim JS
FAU - Han, K-H
AU  - Han KH
FAU - Lee, G T
AU  - Lee GT
FAU - Song, J S
AU  - Song JS
FAU - Chung, S H
AU  - Chung SH
FAU - Jeon, J S
AU  - Jeon JS
FAU - Ha, H
AU  - Ha H
FAU - Lee, H B
AU  - Lee HB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Angiotensin II Type 1 Receptor Blockers)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Angiotensin II Type 1 Receptor Blockers/pharmacology
MH  - Animals
MH  - Male
MH  - *Oxidative Stress/drug effects
MH  - *Peritoneal Dialysis
MH  - Peritoneum/drug effects/*metabolism/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
EDAT- 2006/04/28 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/04/28 09:00
PHST- 2006/04/28 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/04/28 09:00 [entrez]
AID - S0085-2538(15)51424-5 [pii]
AID - 10.1038/sj.ki.5001506 [doi]
PST - ppublish
SO  - Kidney Int. 2006 Jun;69(11):2022-8. doi: 10.1038/sj.ki.5001506.