PMID- 1664195
OWN - NLM
STAT- MEDLINE
DCOM- 19920309
LR  - 20220311
IS  - 0091-7370 (Print)
IS  - 0091-7370 (Linking)
VI  - 21
IP  - 6
DP  - 1991 Nov-Dec
TI  - Sequence homology of deoxyribonucleic acid to mouse mammary tumor virus genome in 
      human breast tumors.
PG  - 402-12
AB  - Fifty-two human breast tumors were screened for the presence of DNA homology to 
      mouse mammary tumor virus (MMTV) using molecularly cloned MMTV proviral genomic 
      DNA probes and dot-blot hybridization. Seven patients were found to contain an 
      entire provirus (gag, pol, env, and LTR positive at high stringency). Fifty 
      percent (5/10) of patients having a first degree relative with breast carcinoma 
      were found to have DNA homology to the gag-pol portion of the MMTV genome when 
      hybridization and washing was performed at moderate (56C) stringency. Thirty-nine 
      percent (7/18) of patients with any positive family history and 23 percent (8/34) 
      of patients with a negative family history demonstrated homology under these 
      parameters. Of the patients positive for gag-pol at moderate stringency, fewer 
      had taken exogenous hormones than the sample group (20 percent vs 52 percent), 
      more were parous (93 percent vs 68 percent), estrogen receptor positive (69 
      percent vs 48 percent), and male (13 percent vs 4 percent). At higher stringency 
      (62C) no correlation to family history, hormone use or sex was detected, but 
      positivity was noted among estrogen and progesterone receptor positive patients 
      (67 percent vs 48 percent). Under lower stringency wash conditions, mismatched 
      MMTV-related sequences are identified suggesting the existence of an endogenous 
      gene with partial homology to MMTV. High stringency hybridization may identify a 
      related retrovirus with significant homology to MMTV.
FAU - Szakacs, J G
AU  - Szakacs JG
AD  - Department of Pathology, H. Lee Moffitt Cancer Center, University of South 
      Florida, Tampa 33612.
FAU - Moscinski, L C
AU  - Moscinski LC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
RN  - 0 (Estrogens)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Breast Neoplasms/chemistry/*genetics/pathology
MH  - DNA, Neoplasm/*analysis
MH  - DNA, Viral/*analysis
MH  - Estrogens/pharmacology
MH  - Female
MH  - Genes, gag
MH  - Genes, pol
MH  - *Genome, Viral
MH  - Humans
MH  - Male
MH  - Mammary Tumor Virus, Mouse/*genetics/ultrastructure
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Middle Aged
MH  - Progesterone/pharmacology
MH  - Proviruses/genetics
MH  - Reference Standards
MH  - *Sequence Homology, Nucleic Acid
MH  - Sex Factors
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
PST - ppublish
SO  - Ann Clin Lab Sci. 1991 Nov-Dec;21(6):402-12.