PMID- 16643803
OWN - NLM
STAT- MEDLINE
DCOM- 20090608
LR  - 20201209
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 22
IP  - 3
DP  - 2006 May
TI  - [Expression of mouse lipopolysaccharide response protein LRP in E. coli and 
      preparation of rabbit anti-mLRP serum].
PG  - 371-3, 376
AB  - AIM: To express mouse lipopolysaccharide response protein (mLRP) and prepare 
      rabbit anti-mLRP serum. METHODS: The predicted mouse lrp cDNA sequence was 
      obtained by splicing homologous ESTs by comparing human lrp cDNA with mouse ESTs. 
      Then the primers were designed. mlrp cDNA from NIH3T3 cells stimulated with 
      lipopolysaccharide (LPS) was amplified by RT-PCR and was cloned into prokaryotic 
      expression vector pTAT to construct recombinant expression vector pTAT-mlrp. The 
      His-TAT-mLRP fusion protein was expressed in E. coli BL21(DE3) and was used to 
      immunize the rabbits to get rabbit anti-mLRP serum. The anti-serum was purified 
      by the acetone precipitation method. The specificity of the rabbit anti-mLRP 
      serum was determined by Western blot. RESULTS: The predicted length of mlrp cDNA 
      was 1905 bp. The encoding region of the cloned mlrp cDNA, 1554 bp, was inserted 
      into pTAT. The His-TAT-mLRP fusion protein was expressed successfully in E. coli. 
      The rabbit anti-mLRP serum was prepared by immunizing the rabbit with mLRP 
      protein. CONCLUSION: The successful expression of mLRP and the preparation of 
      rabbit anti-mLRP serum lays the foundation for further study of the function of 
      mLRP.
FAU - Dai, Zhong-ming
AU  - Dai ZM
AD  - Department of Biochemistry and Molecular Biology, Fourth Military Medical 
      University, Xi'an 710032, China. daizmly@yahoo.com
FAU - Chen, Su-min
AU  - Chen SM
FAU - Du, Ke-jun
AU  - Du KJ
FAU - Chen, Nan-chun
AU  - Chen NC
FAU - Song, Qing-he
AU  - Song QH
FAU - Luo, Wen-jing
AU  - Luo WJ
FAU - Chen, Jing-yuan
AU  - Chen JY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (lipopolysaccharide-binding protein)
SB  - IM
MH  - Acute-Phase Proteins/genetics/*immunology/metabolism
MH  - Animals
MH  - Antibody Formation/*immunology
MH  - Carrier Proteins/genetics/*immunology/metabolism
MH  - Cloning, Molecular
MH  - Escherichia coli/*genetics
MH  - Female
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Lipopolysaccharides/chemistry
MH  - Membrane Glycoproteins/genetics/*immunology/metabolism
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Rabbits
MH  - Recombinant Proteins/genetics/*immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/04/29 09:00
MHDA- 2009/06/09 09:00
CRDT- 2006/04/29 09:00
PHST- 2006/04/29 09:00 [pubmed]
PHST- 2009/06/09 09:00 [medline]
PHST- 2006/04/29 09:00 [entrez]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 May;22(3):371-3, 376.