PMID- 16644919
OWN - NLM
STAT- MEDLINE
DCOM- 20071221
LR  - 20220408
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 24
IP  - 8
DP  - 2006 Aug
TI  - Differentiation of human embryonic stem cells into bipotent mesenchymal stem 
      cells.
PG  - 1914-22
AB  - Mesenchymal stem cells (MSCs) are multipotent progenitors that can be found in 
      many connective tissues, including fat, bone, cartilage, and muscle. We report 
      here a method to reproducibly differentiate human embryonic stem cells (hESCs) 
      into MSCs that does not require the use of any feeder layer. The cells obtained 
      with this procedure are morphologically similar to bone marrow MSCs, are 
      contact-inhibited, can be grown in culture for about 20 to 25 passages, have an 
      immunophenotype similar to bone marrow MSCs (negative for CD34 and CD45 and 
      positive for CD13, CD44, CD71, CD73, CD105, CD166, human leukocyte antigen 
      [HLA]-ABC, and stage-specific embryonic antigen [SSEA]-4), can differentiate into 
      osteocytes and adipocytes, and can be used as feeder cells to support the growth 
      of undifferentiated hESCs. The ability to produce MSCs from hESCs should prove 
      useful to produce large amounts of genetically identical and genetically 
      modifiable MSCs that can be used to study the biology of MSCs and for therapeutic 
      applications.
FAU - Olivier, Emmanuel N
AU  - Olivier EN
AD  - Department of Medicine, Division of Hematology, Einstein Center for Human 
      Embryonic Stem Cell Research, Albert Einstein College of Medicine, 1300 Morris 
      Park Avenue, Bronx, New York 10461, USA.
FAU - Rybicki, Anne C
AU  - Rybicki AC
FAU - Bouhassira, Eric E
AU  - Bouhassira EE
LA  - eng
GR  - HL-38655/HL/NHLBI NIH HHS/United States
GR  - HL-68962/HL/NHLBI NIH HHS/United States
GR  - P01-HL55435/HL/NHLBI NIH HHS/United States
GR  - P20-GM075037/GM/NIGMS NIH HHS/United States
GR  - R01-DK56845/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060427
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Culture Media)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cells, Cultured
MH  - Culture Media/pharmacology
MH  - Embryonic Stem Cells/*cytology/drug effects/physiology
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects/physiology
MH  - RNA/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
EDAT- 2006/04/29 09:00
MHDA- 2007/12/22 09:00
CRDT- 2006/04/29 09:00
PHST- 2006/04/29 09:00 [pubmed]
PHST- 2007/12/22 09:00 [medline]
PHST- 2006/04/29 09:00 [entrez]
AID - 2005-0648 [pii]
AID - 10.1634/stemcells.2005-0648 [doi]
PST - ppublish
SO  - Stem Cells. 2006 Aug;24(8):1914-22. doi: 10.1634/stemcells.2005-0648. Epub 2006 
      Apr 27.