PMID- 16645199
OWN - NLM
STAT- MEDLINE
DCOM- 20060804
LR  - 20211020
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 8
IP  - 2
DP  - 2006 May
TI  - FISH analysis for the detection of lymphoma-associated chromosomal abnormalities 
      in routine paraffin-embedded tissue.
PG  - 141-51
AB  - Over the last decade, fluorescence in situ hybridization (FISH) has become a 
      firmly established technique in the diagnosis and assessment of lymphoid 
      malignancies. However, this technique is not wide-ly used in the routine 
      diagnostic evaluation of paraffin-embedded biopsies, most likely because of a 
      perception that it is technically more demanding. There are also uncertainties 
      regarding diagnostic thresholds and the way in which results should be 
      interpreted. In this Review, we describe practical strategies for using FISH 
      analysis to detect lymphoma-associated chromosomal abnormalities in routine 
      paraffin-embedded lymphoma biopsies. Furthermore, we provide proposals on how 
      FISH results should be interpreted (including how to calculate cutoff levels for 
      FISH probes), recorded, and reported. An online appendix (available at 
      http://jmd.amjpathol.org) details various simple, yet robust procedures for 
      paraffin FISH analysis; it also provides additional information on the production 
      of FISH probes, evaluating and reporting FISH results, sources for reagents and 
      equipment, and troubleshooting. We hope that these suggestions will make FISH 
      technology for the study of lymphoma biopsies more accessible to routine 
      diagnostic and research laboratories.
FAU - Ventura, Roland A
AU  - Ventura RA
AD  - LRF Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, 
      John Radcliffe Hospital, Oxford, United Kingdom.
FAU - Martin-Subero, Jose I
AU  - Martin-Subero JI
FAU - Jones, Margaret
AU  - Jones M
FAU - McParland, Joanna
AU  - McParland J
FAU - Gesk, Stefan
AU  - Gesk S
FAU - Mason, David Y
AU  - Mason DY
FAU - Siebert, Reiner
AU  - Siebert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
SB  - IM
MH  - *Chromosome Aberrations
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Lymphoma/*genetics/metabolism/*pathology
MH  - Paraffin Embedding/*methods
PMC - PMC1867591
EDAT- 2006/04/29 09:00
MHDA- 2006/08/05 09:00
PMCR- 2007/05/01
CRDT- 2006/04/29 09:00
PHST- 2006/04/29 09:00 [pubmed]
PHST- 2006/08/05 09:00 [medline]
PHST- 2006/04/29 09:00 [entrez]
PHST- 2007/05/01 00:00 [pmc-release]
AID - S1525-1578(10)60712-X [pii]
AID - 6392 [pii]
AID - 10.2353/jmoldx.2006.050083 [doi]
PST - ppublish
SO  - J Mol Diagn. 2006 May;8(2):141-51. doi: 10.2353/jmoldx.2006.050083.