PMID- 16645955
OWN - NLM
STAT- MEDLINE
DCOM- 20060830
LR  - 20131121
IS  - 1522-7235 (Print)
IS  - 1522-7235 (Linking)
VI  - 21
IP  - 3
DP  - 2006 May-Jun
TI  - Improvement of the acridine orange-protein-surfactant system for protein 
      estimation based on aromatic ring stacking effect of sodium dodecyl benzene 
      sulphonate.
PG  - 186-94
AB  - The fluorescence of acridine orange (AO) is greatly quenched by the anionic 
      surfactant sodium dodecyl benzene sulphonate (SDBS), but when protein is added 
      into the AO-SDBS system, the fluorescence intensity of the latter is enhanced 
      again. Based on this, a new fluorimetric method of determination of protein was 
      developed. Under optimum conditions, the enhanced intensity of fluorescence is in 
      proportion to the concentration of protein, such as bovine serum albumin (BSA), 
      human serum albumin (HSA) and egg albumin (EA), over a wide range with detection 
      limits at the 10(-9) g/mL level. This method has been satisfactorily used for the 
      determination of protein in samples. We compared results using 280 nm and 490 nm 
      excitation wavelengths and the mechanism of the assay.
CI  - Copyright (c) 2006 John Wiley & Sons, Ltd.
FAU - Wang, Fei
AU  - Wang F
AD  - Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry 
      of Education, School of Chemistry and Chemical Engineering, Shandong University, 
      Jinan 250100, People's Republic of China.
FAU - Yang, Jinghe
AU  - Yang J
FAU - Wu, Xia
AU  - Wu X
FAU - Wang, Xiaobo
AU  - Wang X
FAU - Guo, Changying
AU  - Guo C
FAU - Jia, Zhen
AU  - Jia Z
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Benzenesulfonates)
RN  - 0 (Buffers)
RN  - 0 (Hydrocarbons, Aromatic)
RN  - 0 (Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (Surface-Active Agents)
RN  - 60NSK897G9 (dodecylbenzenesulfonic acid)
RN  - 9006-59-1 (Ovalbumin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Animals
MH  - Benzenesulfonates/*chemistry
MH  - Buffers
MH  - Humans
MH  - Hydrocarbons, Aromatic/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Ovalbumin/analysis/chemistry
MH  - Proteins/*analysis/chemistry
MH  - Scattering, Radiation
MH  - Serum Albumin/analysis/chemistry
MH  - Spectrometry, Fluorescence
MH  - Surface-Active Agents/*chemistry
EDAT- 2006/04/29 09:00
MHDA- 2006/08/31 09:00
CRDT- 2006/04/29 09:00
PHST- 2006/04/29 09:00 [pubmed]
PHST- 2006/08/31 09:00 [medline]
PHST- 2006/04/29 09:00 [entrez]
AID - 10.1002/bio.904 [doi]
PST - ppublish
SO  - Luminescence. 2006 May-Jun;21(3):186-94. doi: 10.1002/bio.904.