PMID- 16646074
OWN - NLM
STAT- MEDLINE
DCOM- 20060921
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 119
IP  - 7
DP  - 2006 Oct 1
TI  - Multi-target fluorescence in situ hybridization in bladder washings for 
      prediction of recurrent bladder cancer.
PG  - 1660-5
AB  - The objective of this study was to evaluate the diagnostic value of chromosomal 
      analysis by fluorescence in situ hybridization (FISH) for predicting recurrence 
      of urothelial carcinoma (UC) after transurethral resection. One hundred and 
      thirty-eight patients (median age 68.5 years) with a history of UC were eligible 
      for this prospective study. FISH was applied to cytospin specimens prepared from 
      bladder washings taken during a negative control cystoscopy. The multi-target 
      FISH test UroVysion (Abbott/Vysis) containing probes to the centromeres of 
      chromosomes 3, 7, 17 and the 9p21 locus was used. UC recurrence was defined as a 
      positive biopsy during follow-up. The median follow-up time was 19.2 (4-52) 
      months. FISH was positive in 50 (36%) patients and negative in 88 (64%) patients. 
      A recurrence occurred in 39% of the patients with a positive FISH test and in 21% 
      of patients with a negative FISH test. FISH positivity according to 
      manufacturer's criteria, at the time of a negative cystoscopy, was not 
      significantly associated with the risk of recurrence (p = 0.12). However, the 
      sensitivity of the FISH test to predict recurrence was significantly improved by 
      considering specimens with rare (< or =10) tetraploid cells as negative (p < 
      0.006). In addition, presence of 9p21 deletion was significantly associated with 
      recurrence (p < 0.01). Notably, positive standard cytology was an independent 
      factor for subsequent recurrence in this study (p < 0.001). Taken together, 
      multi-target FISH may help to stratify the risk of recurrence of UC at the time 
      of a negative follow-up cystoscopy. Defining the optimal threshold for FISH 
      positivity requires consideration of tetraploid pattern and 9p21 deletion. Our 
      results also emphasize the paramount importance of conventional cytology for UC 
      surveillance.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Zellweger, Tobias
AU  - Zellweger T
AD  - Department of Urology, University of Basel, Switzerland.
FAU - Benz, Gabriel
AU  - Benz G
FAU - Cathomas, Gieri
AU  - Cathomas G
FAU - Mihatsch, Michael J
AU  - Mihatsch MJ
FAU - Sulser, Tullio
AU  - Sulser T
FAU - Gasser, Thomas C
AU  - Gasser TC
FAU - Bubendorf, Lukas
AU  - Bubendorf L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human/genetics
MH  - Disease-Free Survival
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/*genetics/*pathology/urine
MH  - Urinary Bladder Neoplasms/*genetics/*pathology/urine
EDAT- 2006/04/29 09:00
MHDA- 2006/09/22 09:00
CRDT- 2006/04/29 09:00
PHST- 2006/04/29 09:00 [pubmed]
PHST- 2006/09/22 09:00 [medline]
PHST- 2006/04/29 09:00 [entrez]
AID - 10.1002/ijc.21704 [doi]
PST - ppublish
SO  - Int J Cancer. 2006 Oct 1;119(7):1660-5. doi: 10.1002/ijc.21704.