PMID- 16646463
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20190715
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 40
IP  - 7
DP  - 2006 Apr 1
TI  - Culture-based MEMS device to track Gordonia in activated sludge.
PG  - 2269-74
AB  - Previously, we reported on the use of microelectromechanical systems (MEMS) 
      fabrication technologies to develop paraffin surfaces for miniaturization of 
      culture-based detection and rapid quantification of Mycolata in environmental 
      samples. In the current study, the novel culture-based biochip was calibrated 
      with a broad range of pure cultures of Mycolata including Gordonia spp. isolated 
      from activated sludge foam. The biochip successfully recovered Gordonia amarae 
      spiked into a sample of mixed liquor collected from a municipal activated sludge 
      system. Comparisons of these results with molecular biology-based assays 
      including 16S rRNA-targeted fluorescence in situ hybridization (FISH) and 
      antibody staining demonstrated that the biochip provides a more rapid and 
      user-friendly platform for reliable identification and quantification of Mycolata 
      in full-scale municipal activated sludge sewage treatment plants. The results of 
      this work successfully demonstrate an alternative platform technology for 
      inexpensive monitoring of environmental microorganisms using existing expertise 
      by potential users in the area of bacterial cultivation.
FAU - Polaczyk, Amy
AU  - Polaczyk A
AD  - Department of Civil and Environmental Engineering, University of Cincinnati, 
      Cincinnati, Ohio 45221-0071, USA.
FAU - Kinkle, Brian
AU  - Kinkle B
FAU - Papautsky, Ian
AU  - Papautsky I
FAU - Oerther, Daniel B
AU  - Oerther DB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Calibration
MH  - Gordonia Bacterium/genetics/*isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/analysis
MH  - Sewage/*microbiology
EDAT- 2006/05/02 09:00
MHDA- 2006/08/25 09:00
CRDT- 2006/05/02 09:00
PHST- 2006/05/02 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2006/05/02 09:00 [entrez]
AID - 10.1021/es052071+ [doi]
PST - ppublish
SO  - Environ Sci Technol. 2006 Apr 1;40(7):2269-74. doi: 10.1021/es052071+.