PMID- 1664738
OWN - NLM
STAT- MEDLINE
DCOM- 19920320
LR  - 20190918
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 3
IP  - 6
DP  - 1991
TI  - Dual effects of ATP on phosphatidylinositol breakdown in rat hepatocyte 
      membranes.
PG  - 577-85
AB  - The mechanisms whereby adenosine-5'-triphosphate (ATP) regulates the inositol 
      phospholipid-signalling system were studied in rat hepatocytes. Intact 
      hepatocytes respond to extracellular ATP, adenosine-5'-O-(3-thiotriphosphate) 
      (ATP gamma S), ADP and weakly to guanosine-5'-triphosphate (GTP), but not to 
      other purine nucleotides (GDP or AMP). This is consistent with the idea that a P2 
      purinergic receptor is coupled to the phosphatidylinositol metabolism in these 
      cells. Partially purified plasma membranes prepared from myo-[3H]inositol 
      prelabelled hepatocytes exhibit a phosphatidylinositol-4,5-bisphosphate 
      phospholipase C activity sensitive to ATP, ATP gamma S and 
      guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S). Moreover the GTP gamma S 
      effect is greatly enhanced by ATP and ATP gamma S. These potentiating effects 
      differ according to the adenylnucleotide considered. ATP produces (1) an increase 
      in the GTP gamma S-PLC sensitivity, (2) a potentiation of the phospholipase C 
      (PLC) response induced by maximal dose of GTP gamma S, and (3) an increase in the 
      inositol lipids pools. At variance, ATP gamma S, a nonhydrolysable analogue of 
      ATP, only increases the PLC-sensitivity towards GTP gamma S. These results may 
      signify that ATP stimulates inositol phosphate accumulation via at least two 
      distinct mechanisms (i) a direct activation of a P2 purinergic receptor coupled 
      to a PLC via a GTP binding protein and (ii) a stimulation of the 
      phosphatidylinositol (PI) and phosphatidylinositol-4-phosphate (PIP) kinases 
      which increased the pool of phospholipase C substrates.
FAU - Ibarrondo, J
AU  - Ibarrondo J
AD  - Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, 
      Universidad del Pais Vasco/Euskal Herriko Unibertsitatea, Bilabo, Spain.
FAU - Marino, A
AU  - Marino A
FAU - Guillon, G
AU  - Guillon G
FAU - Trueba, M
AU  - Trueba M
FAU - Macarulla, J M
AU  - Macarulla JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Phosphatidylinositols)
RN  - 35094-46-3 (adenosine 5'-O-(3-thiotriphosphate))
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
MH  - Adenosine Triphosphate/analogs & derivatives/*pharmacology
MH  - Animals
MH  - Cell Membrane/drug effects/metabolism
MH  - Enzyme Activation
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Liver/cytology/drug effects/*metabolism
MH  - Male
MH  - Phosphatidylinositols/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Type C Phospholipases/*metabolism
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0898-6568(91)90034-R [pii]
AID - 10.1016/0898-6568(91)90034-r [doi]
PST - ppublish
SO  - Cell Signal. 1991;3(6):577-85. doi: 10.1016/0898-6568(91)90034-r.