PMID- 1664775 OWN - NLM STAT- MEDLINE DCOM- 19920323 LR - 20190613 IS - 0006-8993 (Print) IS - 0006-8993 (Linking) VI - 563 IP - 1-2 DP - 1991 Nov 1 TI - Separation of alpha 1, alpha 2 and alpha 3 subunits of the GABAA-benzodiazepine receptor complex by immunoaffinity chromatography. PG - 325-8 AB - Polyclonal antibodies raised to synthetic amino acid sequences of the rat gamma-aminobutyric acid (GABAA) receptor alpha 1-, alpha 2- and alpha 3-subunits selectively recognized single proteins with apparent molecular weight 51 kDa (P51), 53 kDa (P53) and 59 kDa (P59), respectively, in GABAA receptor preparations affinity purified from the brains of 5-10-day-old rats. The antibodies were coupled to Affigel 10, and the resulting immunoaffinity columns were used to isolate these proteins from affinity purified GABAA receptors. FAU - Zezula, J AU - Zezula J AD - Department of Biochemical Psychiatry, Universitatsklinik fur Psychiatrie, Vienna, Austria. FAU - Fuchs, K AU - Fuchs K FAU - Sieghart, W AU - Sieghart W LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Peptides) RN - 0 (Receptors, GABA-A) SB - IM MH - Amino Acid Sequence MH - Animals MH - Blotting, Western MH - Chromatography, Affinity MH - Molecular Sequence Data MH - Molecular Weight MH - Peptides/analysis MH - Rats MH - Receptors, GABA-A/*chemistry/immunology EDAT- 1991/11/01 00:00 MHDA- 1991/11/01 00:01 CRDT- 1991/11/01 00:00 PHST- 1991/11/01 00:00 [pubmed] PHST- 1991/11/01 00:01 [medline] PHST- 1991/11/01 00:00 [entrez] AID - 0006-8993(91)91556-G [pii] AID - 10.1016/0006-8993(91)91556-g [doi] PST - ppublish SO - Brain Res. 1991 Nov 1;563(1-2):325-8. doi: 10.1016/0006-8993(91)91556-g.