PMID- 16648150
OWN - NLM
STAT- MEDLINE
DCOM- 20060818
LR  - 20161124
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 12
IP  - 6
DP  - 2006 Jun
TI  - Tyrosine kinase B receptor and its activated neurotrophins in ovaries from human 
      fetuses and adults.
PG  - 357-65
AB  - The signals initiating the growth of primordial follicles are unknown. Growth 
      factors such as neurotrophin 4/5 (NT-4/5) and brain-derived neurotrophic factor 
      (BDNF) may play a role in this process. To investigate the expression of NT-4/5 
      and BDNF and their receptor tyrosine kinase B (TrkB) in the early developing 
      follicles, we fixed and froze 12 ovarian samples from adolescents/adults and 31 
      ovaries from human fetuses. The fixed samples were prepared for 
      immunohistochemical staining for NT-4/5, BDNF and the TrkB receptor. Total RNA 
      was extracted from the frozen ovarian samples, and the expression of NT-4/5, BDNF 
      and the TrkB receptor (full length and two truncated isoforms) was investigated 
      by RT-PCR. Products were resolved by 1% agarose gel electrophoresis and image 
      analysis. Immunohistochemical staining revealed the expression of NT-4/5 and BDNF 
      mainly in oocytes and, in a minority of samples, also in the granulosa cells 
      (GCs); TrkB receptor was identified in oocytes and GCs. Transcripts of NT-4/5, 
      BDNF and all forms of TrkB receptor were identified in the samples. To elucidate 
      whether indeed NT-4/5 and BDNF are involved in growth initiation of human 
      primordial follicles, they should be added to the culture medium.
FAU - Harel, S
AU  - Harel S
AD  - Infertility and IVF UnitHelen Schneider's Women Hospital, Rabin Medical Center, 
      Petach Tikva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 
      Israel.
FAU - Jin, S
AU  - Jin S
FAU - Fisch, B
AU  - Fisch B
FAU - Feldberg, D
AU  - Feldberg D
FAU - Krissi, H
AU  - Krissi H
FAU - Felz, C
AU  - Felz C
FAU - Freimann, S
AU  - Freimann S
FAU - Tan, S L
AU  - Tan SL
FAU - Ao, A
AU  - Ao A
FAU - Abir, R
AU  - Abir R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060428
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 145172-44-7 (neurotrophin 5)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics
MH  - Female
MH  - Fetus
MH  - Humans
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/*analysis/genetics
MH  - Ovarian Follicle/chemistry/cytology/metabolism
MH  - Ovary/*chemistry/cytology/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptor, trkB/*analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/05/02 09:00
MHDA- 2006/08/19 09:00
CRDT- 2006/05/02 09:00
PHST- 2006/05/02 09:00 [pubmed]
PHST- 2006/08/19 09:00 [medline]
PHST- 2006/05/02 09:00 [entrez]
AID - gal033 [pii]
AID - 10.1093/molehr/gal033 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2006 Jun;12(6):357-65. doi: 10.1093/molehr/gal033. Epub 2006 Apr 
      28.