PMID- 16648298
OWN - NLM
STAT- MEDLINE
DCOM- 20060629
LR  - 20181217
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 189
IP  - 2
DP  - 2006 May
TI  - Enhanced expression of hexokinase I in pancreatic islets induced by sucrose 
      administration.
PG  - 311-7
AB  - Administration of a sucrose-rich diet (SRD) to normal hamsters induces an 
      insulin-resistant state and a significant increase of insulin secretion and 
      beta-cell mass. Islets isolated from these animals had a marked increase in 
      glucose metabolism and glucose-induced insulin secretion, at both low and high 
      glucose concentrations. They also presented increased hexokinase (HK) activity, 
      without measurable changes in glucokinase (GK) activity. In this study we 
      measured HK and GK activity in homogenates of islets isolated from normal control 
      and SRD-fed hamsters, as well as in their particulate and cytosolic fractions. We 
      also measured transcription rate (mRNA by reverse transcriptase PCR) and 
      expression levels (Western blotting) of both enzymes in these islets. We found an 
      increase in HK activity and expression levels, without measurable changes in HK 
      mRNA level in SRD-fed animals. Whereas a similar GK activity was measured in 
      homogenates of islets isolated from both groups, such activity was significantly 
      higher in the cytosolic fraction of SRD islets. On the other hand, GK 
      transcription rate and expression level were similar in both experimental groups. 
      Our results suggest that the increased beta-cell secretory response to low 
      glucose can be partly ascribed to an increased activity of islet HK consecutive 
      to an enhanced expression of the enzyme, while the enhanced response to high 
      glucose could be due to changes in GK compartmentalization.
FAU - Maiztegui, B
AU  - Maiztegui B
AD  - CENEXA-Center of Experimental and Applied Endocrinology (UNLP-CONICET, PAHO/WHO 
      Collaborating Center), National University of La Plata School of Medicine, 60 y 
      120 1900 La Plata, Argentina.
FAU - Borelli, M I
AU  - Borelli MI
FAU - Massa, M L
AU  - Massa ML
FAU - Del Zotto, H
AU  - Del Zotto H
FAU - Gagliardino, J J
AU  - Gagliardino JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 57-50-1 (Sucrose)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Blotting, Western/methods
MH  - Body Weight/physiology
MH  - Cricetinae
MH  - Cytosol/metabolism
MH  - Diet
MH  - Drinking/physiology
MH  - Gene Expression/genetics
MH  - Glucokinase/analysis/genetics/*metabolism
MH  - Hexokinase/analysis/genetics/*metabolism
MH  - Insulin/blood/metabolism
MH  - Insulin Secretion
MH  - Insulin-Secreting Cells/metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Male
MH  - Mesocricetus
MH  - Phosphorylation
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Sucrose/*administration & dosage
MH  - Transcription, Genetic/genetics
EDAT- 2006/05/02 09:00
MHDA- 2006/06/30 09:00
CRDT- 2006/05/02 09:00
PHST- 2006/05/02 09:00 [pubmed]
PHST- 2006/06/30 09:00 [medline]
PHST- 2006/05/02 09:00 [entrez]
AID - 189/2/311 [pii]
AID - 10.1677/joe.1.06662 [doi]
PST - ppublish
SO  - J Endocrinol. 2006 May;189(2):311-7. doi: 10.1677/joe.1.06662.