PMID- 16650923
OWN - NLM
STAT- MEDLINE
DCOM- 20060927
LR  - 20211020
IS  - 0301-4622 (Print)
IS  - 1873-4200 (Electronic)
IS  - 0301-4622 (Linking)
VI  - 123
IP  - 1
DP  - 2006 Aug 20
TI  - The "interceptor" properties of chlorophyllin measured within the three-component 
      system: intercalator-DNA-chlorophyllin.
PG  - 11-9
AB  - In aqueous solutions, in the presence of double-stranded DNA, chlorophyllin (CHL) 
      forms complexes with each of the three DNA intercalators: acridine orange (AO), 
      quinacrine mustard (QM), and doxorubicin (DOX). The evidence for these 
      interactions was obtained by measurement changes in the absorption and 
      fluorescence spectra of the mixtures containing DNA and intercalators during 
      titration with CHL. A model of simple competition between DNA and CHL for the 
      intercalator was used to define the measured interactions. The concentrations of 
      the complexes estimated based on this model were consistent with the 
      concentrations obtained by actual measurement of the absorption spectra. The 
      present data provide further support for the role of chlorophyllin as an 
      "interceptor" that may neutralize biological activity of aromatic compounds 
      including mutagens and antitumor drugs.
FAU - Pietrzak, Monika
AU  - Pietrzak M
AD  - Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, 
      Oczapowskiego 4, 10-719 Olsztyn, Poland.
FAU - Wieczorek, Zbigniew
AU  - Wieczorek Z
FAU - Wieczorek, Jolanta
AU  - Wieczorek J
FAU - Darzynkiewicz, Zbigniew
AU  - Darzynkiewicz Z
LA  - eng
GR  - R01 CA028704/CA/NCI NIH HHS/United States
GR  - R01 CA028704-27/CA/NCI NIH HHS/United States
GR  - CA 28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060406
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Chlorophyllides)
RN  - 0 (Intercalating Agents)
RN  - 9007-49-2 (DNA)
RN  - EEM82VOY7C (chlorophyllin)
SB  - IM
MH  - Chlorophyllides/*chemistry
MH  - DNA/*chemistry
MH  - Intercalating Agents/*chemistry
MH  - Spectrometry, Fluorescence
PMC - PMC3488280
MID - NIHMS76826
EDAT- 2006/05/03 09:00
MHDA- 2006/09/28 09:00
PMCR- 2012/11/03
CRDT- 2006/05/03 09:00
PHST- 2006/01/13 00:00 [received]
PHST- 2006/03/28 00:00 [revised]
PHST- 2006/03/30 00:00 [accepted]
PHST- 2006/05/03 09:00 [pubmed]
PHST- 2006/09/28 09:00 [medline]
PHST- 2006/05/03 09:00 [entrez]
PHST- 2012/11/03 00:00 [pmc-release]
AID - S0301-4622(06)00101-3 [pii]
AID - 10.1016/j.bpc.2006.03.018 [doi]
PST - ppublish
SO  - Biophys Chem. 2006 Aug 20;123(1):11-9. doi: 10.1016/j.bpc.2006.03.018. Epub 2006 
      Apr 6.