PMID- 16651390
OWN - NLM
STAT- MEDLINE
DCOM- 20060919
LR  - 20161124
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 54
IP  - 9
DP  - 2006 Sep
TI  - Differential expression of cell surface markers by ovine respiratory tract 
      dendritic cells.
PG  - 1021-30
AB  - Dendritic cells (DCs) are key antigen-presenting cells central to the induction 
      of primary immune responses. Despite the prevalence of respiratory disease in 
      sheep and the increasing use of the ovine lung as a model for human disease, 
      ovine respiratory tract DCs (RTDCs) have not yet been characterized. Using single 
      and double immunocytochemical staining, expression of a number of potential DC 
      markers (MHC class II, CD1b, SIRPalpha, and CD205) by ovine RTDC populations has 
      been determined. MHC class II staining revealed widespread populations of DCs 
      either adjacent to respiratory airway epithelium or within the lung parenchyma. 
      CD1b was expressed by a small subpopulation of both airway and parenchymal RTDCs. 
      Expression of SIRPalpha was limited to a small subpopulation of airway RTDCs but 
      was absent from the lung parenchyma. CD205 was widely expressed by airway RTDCs 
      but expressed only by a small subpopulation of parenchymal RTDCs. In addition, 
      the majority (87%) of parenchymal CD205+ cells exhibited a non-DC-like morphology 
      and did not express MHC class II, suggesting that these single CD205+ cells were 
      not DCs. Phenotypic differences between airway and parenchymal RTDCs may be 
      related to functional differences between the two populations.
FAU - McNeilly, Tom N
AU  - McNeilly TN
AD  - Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush 
      VEterinary Centre, Midlothian, UK. Tom.McNeilly@Moredun.ac.uk
FAU - Brown, Jeremy K
AU  - Brown JK
FAU - Harkiss, Gordon
AU  - Harkiss G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060501
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
RN  - 0 (CD1b antigen)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/biosynthesis
MH  - Antigens, CD1/biosynthesis
MH  - Antigens, Surface/*biosynthesis
MH  - Biomarkers/metabolism
MH  - Bronchi/metabolism
MH  - Dendritic Cells/*metabolism
MH  - Female
MH  - Genes, MHC Class II
MH  - Histocompatibility Antigens Class II/biosynthesis
MH  - Immunohistochemistry
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Lectins, C-Type/biosynthesis
MH  - Lung/metabolism
MH  - Minor Histocompatibility Antigens
MH  - Organ Specificity
MH  - Receptors, Cell Surface/biosynthesis
MH  - Respiratory Mucosa/metabolism
MH  - Respiratory System/*metabolism
MH  - Sheep
MH  - Trachea/metabolism
EDAT- 2006/05/03 09:00
MHDA- 2006/09/20 09:00
CRDT- 2006/05/03 09:00
PHST- 2006/05/03 09:00 [pubmed]
PHST- 2006/09/20 09:00 [medline]
PHST- 2006/05/03 09:00 [entrez]
AID - jhc.6A6940.2006 [pii]
AID - 10.1369/jhc.6A6940.2006 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2006 Sep;54(9):1021-30. doi: 10.1369/jhc.6A6940.2006. Epub 
      2006 May 1.