PMID- 16653098
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100629
LR  - 20211020
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 100
IP  - 3
DP  - 1992 Nov
TI  - Plasma membrane vesicles from source and sink leaves : changes in solute 
      transport and polypeptide composition.
PG  - 1150-6
AB  - Plasma membrane vesicles (PMVs) were prepared by phase partitioning from 
      microsomal fractions of either sink or source leaves of sugar beet (Beta vulgaris 
      L.). The purity, the internal volume, the sidedness, and the sealingness of PMVs 
      prepared from sink leaves did not differ from those measured with PMVs from 
      source leaves. Yet, in response to an imposed proton motive force, PMVs from 
      source leaves accumulated about 4-fold more sucrose than PMVs from sink leaves. 
      The developmental stage did not affect the uptake of glucose and valine in PMVs 
      prepared from leaf tissues. It was concluded that the sink/source transition is 
      accompanied either by the incorporation into the plasma membrane of leaf cells of 
      proteins mediating proton-sucrose cotransport, or by their activation. 
      N-ethylmaleimide and a polyclonal ascitic fluid directed against the 42-kD region 
      of the plasma membrane containing a putative sucrose carrier inhibited the uptake 
      of sucrose in PMVs from source leaves, but not in PMVs from sink leaves. Sodium 
      dodecyl sulfate gel electrophoresis and western blot suggested that the 42 
      polypeptide was more abundant in the PMVs from source leaves than in the PMVs 
      from sink leaves.
FAU - Lemoine, R
AU  - Lemoine R
AD  - Laboratoire de Physiologie et Biochimie Vegetales, Unite Associee Centre National 
      de la Recherche Scientifique 574, Universite de Poitiers, 25 rue du Faubourg 
      Saint-Cyprien, 86000 Poitiers, France.
FAU - Gallet, O
AU  - Gallet O
FAU - Gaillard, C
AU  - Gaillard C
FAU - Frommer, W
AU  - Frommer W
FAU - Delrot, S
AU  - Delrot S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
PMC - PMC1075759
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
PMCR- 1993/11/01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
PHST- 1993/11/01 00:00 [pmc-release]
AID - 10.1104/pp.100.3.1150 [doi]
PST - ppublish
SO  - Plant Physiol. 1992 Nov;100(3):1150-6. doi: 10.1104/pp.100.3.1150.