PMID- 1665673
OWN - NLM
STAT- MEDLINE
DCOM- 19920409
LR  - 20131121
IS  - 0001-5334 (Print)
IS  - 0001-5334 (Linking)
VI  - 24
IP  - 4
DP  - 1991 Dec
TI  - [Induced differentiation of mouse embryonic stem cells by retinoic acid and 
      dibutyryl cyclic adenosine monophosphate].
PG  - 353-67
AB  - In vitro induced differentiation of mouse embryonic stem cells (ES-5 cells), 
      derived from 5-day 129 mouse blastocyst was studied with retinoic acid (RA) and 
      dibutyryl cyclic adenosine monophosphate (dB-cAMP). RA only or RA with dBcAMP 
      together can both induce monolayer ES-5 cells to differentiate into cells of two 
      types: neuron-like cells and fibroblast-like cells. After treated with 
      10(-6)mol/L RA for 6 days, the differentiated cells were about 80% of all cells, 
      among which most cells were fibroblast-like cells and others were neuron-like 
      cells. While after 6 days of treatment with 10(-6)mol/L RA and 1 mmol/L dBcAMP, 
      the ratio of differentiated cells can be up to 90-95%, and most cells (about 
      90-95% of differentiated cells) are neuron-like cells. Immunocytochemical 
      analysis of phenotypic markers, especially GFAP and laminin, showed that the 
      neuron-like cells were glia cells. DBcAMP affected the direction and efficiency 
      of induction by RA. The induced differentiation by RA on attached aggregated ES-5 
      cells was studied as well. In this case, more cell types appeared, such as 
      epitheloid cells, fibroblast-like cells and spindle shaped cells and so on. The 
      exact nature of these differentiated cells was not identified. After attached 
      culture for about 15 days, rhythmically contracting cardiac-like muscle cells 
      were most attractive among those several differentiated cell types. The change of 
      phenotypic markers during induced differentiation of ES-5 cells in monolayer and 
      aggregated state was summarized in table 1. Transforming growth factor-beta 1 
      (TGF-beta 1) was also examined in undifferentiated and differentiated cells. 
      Untreated ES-5 cells showed positive immunofluorescent reaction to TGF-beta 1 and 
      various differentiated cells showed different reactions. Glia cells and 
      cardiac-like cells displayed a much stronger TGF-beta 1 reaction. These results 
      indicate that the exact role played by TGF-beta 1 during induced differentiation 
      needs further investigation. The different effect of RA on monolayer and 
      aggregated ES cells and the possible significance of cell to cell interaction in 
      the latter case are discussed.
FAU - Xu, J
AU  - Xu J
AD  - Shanghai Institute of Cell Biology, Academia Sinica.
FAU - Tsung, H C
AU  - Tsung HC
FAU - Yao, Z
AU  - Yao Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Shi Yan Sheng Wu Xue Bao
JT  - Shi yan sheng wu xue bao
JID - 0413570
RN  - 5688UTC01R (Tretinoin)
RN  - 63X7MBT2LQ (Bucladesine)
SB  - IM
MH  - Animals
MH  - Bucladesine/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Embryonic Induction
MH  - Fetus/*cytology
MH  - Mice
MH  - Stem Cells/*drug effects
MH  - Tretinoin/*pharmacology
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
PST - ppublish
SO  - Shi Yan Sheng Wu Xue Bao. 1991 Dec;24(4):353-67.