PMID- 16662530
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100629
LR  - 20240109
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 70
IP  - 2
DP  - 1982 Aug
TI  - Participation of ornithine decarboxylase in early stages of tomato fruit 
      development.
PG  - 540-3
AB  - The apparent association of ornithine decarboxylase (ODC) with rapid cell 
      proliferation in developing tomato (Lycopersicon esculentum Mill. cv. Pearson 
      ms-35) fruits has been previously described. Further evidence is provided by the 
      use of two ODC inhibitors, alpha-difluoromethylornithine (alpha-DFMO) and 
      alpha-methylornithine (alpha-MO). Fruit development was inhibited by these 
      inhibitors if applied during the period of intensive cell division. When applied 
      in vitro, the two inhibitors were shown to inhibit the activity of ODC but not 
      that of arginine decarboxylase (ADC). When applied in vivo, alpha-DFMO, a 
      catalytic irreversible inhibitor, caused 97.1% reduction of ODC activity in the 
      dialyzed extract from the treated ovaries, while it had no effect on ADC. On the 
      other hand, alpha-MO, a reversible inhibitor, did not reduce the activity of 
      these two enzymes in the dialyzed extracts when applied in vivo. The dialysis 
      procedure probably removed alpha-MO from the enzyme fraction. Putrescine, the 
      product of both ODC and ADC, alleviated the inhibition of fruit development but 
      did not restore ODC activity to the control level. These results suggest that in 
      the young developing tomato fruit, ODC is the enzyme responsible for the 
      synthesis of putrescine, which is essential for the early stages of fruit 
      development. The reduced activity of ODC elicited by putrescine suggests a 
      mechanism of feedback regulation by enzyme repression or release of an ODC 
      anti-enzyme.
FAU - Cohen, E
AU  - Cohen E
AD  - Applied Research Institute and Department of Biology, Ben-Gurion University of 
      the Negev, P. O. Box 1025, Beer-Sheva 84110, Israel.
FAU - Arad, S M
AU  - Arad SM
FAU - Heimer, Y M
AU  - Heimer YM
FAU - Mizrahi, Y
AU  - Mizrahi Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
PMC - PMC1067184
EDAT- 1982/08/01 00:00
MHDA- 1982/08/01 00:01
PMCR- 1983/08/01
CRDT- 1982/08/01 00:00
PHST- 1982/08/01 00:00 [pubmed]
PHST- 1982/08/01 00:01 [medline]
PHST- 1982/08/01 00:00 [entrez]
PHST- 1983/08/01 00:00 [pmc-release]
AID - 10.1104/pp.70.2.540 [doi]
PST - ppublish
SO  - Plant Physiol. 1982 Aug;70(2):540-3. doi: 10.1104/pp.70.2.540.