PMID- 16666692
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100629
LR  - 20211020
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 89
IP  - 4
DP  - 1989 Apr
TI  - Evidence that Ribulose 1,5-Bisphosphate (RuBP) Binds to Inactive Sites of RuBP 
      Carboxylase in Vivo and an Estimate of the Rate Constant for Dissociation.
PG  - 1253-7
AB  - The binding of ribulose 1,5-bisphosphate (RuBP) to inactive (noncarbamylated) 
      sites of the enzyme RuBP carboxylase in vivo was investigated in Spinacia 
      oleracea and Helianthus annuus. The concentrations of RuBP and inactive sites 
      were determined in leaf tissue as a function of time after a change to darkness. 
      RuBP concentrations fell rapidly after the change to darkness and were 
      approximately equal to the concentration of inactive sites after 60 s. Variations 
      in the concentration of inactive sites, which were induced by differences in the 
      light intensity before the light-dark transition, correlated with the 
      concentration of RuBP between 60 and 120 s after the change to darkness. These 
      data are discussed as evidence that RuBP binds to inactive sites of RuBP 
      carboxylase in vivo. After the concentration of RuBP fell below that of inactive 
      sites (at times longer than 60 s of darkness), the decline in RuBP was 
      logarithmic with time. This would be expected if the dissociation of RuBP from 
      inactive sites controlled the decline in RuBP concentration. These data were used 
      to estimate the rate constant for dissociation of RuBP from inactive sites in 
      vivo.
FAU - Cardon, Z G
AU  - Cardon ZG
AD  - Biology Department UMC 5305, Utah State University, Logan, Utah 84322.
FAU - Mott, K A
AU  - Mott KA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
PMC - PMC1056004
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
PMCR- 1990/04/01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
PHST- 1990/04/01 00:00 [pmc-release]
AID - 10.1104/pp.89.4.1253 [doi]
PST - ppublish
SO  - Plant Physiol. 1989 Apr;89(4):1253-7. doi: 10.1104/pp.89.4.1253.