PMID- 16670315
OWN - NLM
STAT- MEDLINE
DCOM- 20060622
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 176
IP  - 10
DP  - 2006 May 15
TI  - Induction of primary human T cell responses against hepatitis C virus-derived 
      antigens NS3 or core by autologous dendritic cells expressing hepatitis C virus 
      antigens: potential for vaccine and immunotherapy.
PG  - 6065-75
AB  - Hepatitis C virus (HCV)-specific T cell responses have been suggested to play 
      significant role in viral clearance. Dendritic cells (DCs) are professional APCs 
      that play a major role in priming, initiating, and sustaining strong T cell 
      responses against pathogen-derived Ags. DCs also have inherent capabilities of 
      priming naive T cells against given Ags. Recombinant adenoviral vectors 
      containing HCV-derived Core and NS3 genes were used to endogenously express HCV 
      Core and NS3 proteins in human DCs. These HCV Ags expressing DCs were used to 
      prime and stimulate autologous T cells obtained from uninfected healthy donors. 
      The DCs expressing HCV Core or NS3 Ags were able to stimulate T cells to produce 
      various cytokines and proliferate in HCV Ag-dependent manner. Evidence of both 
      CD4(+) and CD8(+) T cell responses against HCV Core and NS3 generated in vitro 
      were obtained by flow cytometry and Ab blocking experiments. Further, in 
      secondary assays, the T cells primed in vitro exhibited HCV Ag-specific 
      proliferative responses against recombinant protein Ags and also against 
      immunodominant permissive peptide epitopes from HCV Ags. In summary, we 
      demonstrate that the dendritic cells expressing HCV Ags are able to prime the 
      Ag-specific T cells from uninfected healthy individuals in vitro. These studies 
      have implications in designing cellular vaccines, T cell adoptive transfer 
      therapy or vaccine candidates for HCV infection in both prophylactic and 
      therapeutic settings.
FAU - Li, Wen
AU  - Li W
AD  - Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, 
      720 Heritage Medical Research Centre, Edmonton, Alberta, Canada.
FAU - Krishnadas, Deepa K
AU  - Krishnadas DK
FAU - Li, Jie
AU  - Li J
FAU - Tyrrell, D Lorne J
AU  - Tyrrell DL
FAU - Agrawal, Babita
AU  - Agrawal B
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (NS3 protein, hepatitis C virus)
RN  - 0 (Viral Core Proteins)
RN  - 0 (Viral Hepatitis Vaccines)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (nucleocapsid protein, Hepatitis C virus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/metabolism/*virology
MH  - Hepacivirus/*immunology
MH  - Hepatitis C/immunology/*prevention & control/therapy
MH  - Humans
MH  - *Immunotherapy/methods
MH  - Molecular Sequence Data
MH  - T-Lymphocytes/cytology/*immunology/metabolism
MH  - Viral Core Proteins/biosynthesis/*immunology
MH  - Viral Hepatitis Vaccines/*immunology
MH  - Viral Nonstructural Proteins/biosynthesis/*immunology
EDAT- 2006/05/04 09:00
MHDA- 2006/06/23 09:00
CRDT- 2006/05/04 09:00
PHST- 2006/05/04 09:00 [pubmed]
PHST- 2006/06/23 09:00 [medline]
PHST- 2006/05/04 09:00 [entrez]
AID - 176/10/6065 [pii]
AID - 10.4049/jimmunol.176.10.6065 [doi]
PST - ppublish
SO  - J Immunol. 2006 May 15;176(10):6065-75. doi: 10.4049/jimmunol.176.10.6065.