PMID- 16671111
OWN - NLM
STAT- MEDLINE
DCOM- 20060801
LR  - 20071115
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 108
IP  - 3
DP  - 2006 Jun 25
TI  - FISH detection of t(14;18) in follicular lymphoma on Papanicolaou-stained 
      archival cytology slides.
PG  - 198-204
AB  - BACKGROUND: The t(14;18)(q32;q21) translocation is present in about 85% of 
      follicular lymphomas (FL) and can be identified using fluorescence in situ 
      hybridization (FISH). In the diagnostic laboratory setting, the cytologic 
      archival material consists of stained slides, and only rarely is material saved 
      for molecular testing. The authors proposed FISH for FL using 
      Papanicolaou-stained archival cytology material as a practical ancillary 
      technique for diagnosing FL. METHODS: Cases included 35 FL, 6 small lymphocytic 
      lymphomas/chronic lymphocytic leukemias (SLL/CLL), 4 mantle cell lymphomas (MCL), 
      4 marginal zone lymphomas (MZL), 1 lymphoplasmacytic lymphoma (LPL), and 10 
      reactive lymphoid tissues (RLT). FISH was performed on Papanicolaou-stained 
      archival cytology slides using probes for immunoglobulin heavy chain (IGH) on 
      chromosome 14 and BCL2 on chromosome 18. RESULTS: In all, 25 of 32 (81%) FL cases 
      exhibited the t(14;18) translocation, whereas 7 of 32 (19%) lacked the 
      translocation. No cases of non-FL were positive for t(14;18). This series shows a 
      sensitivity of 81% and specificity of 100% for detecting the t(14;18) 
      translocation as a diagnostic tool in FL. CONCLUSIONS: When performed on 
      Papanicolaou-stained cytology slides, FISH for t(14;18) is relatively sensitive 
      and quite specific for FL. These findings are similar to those reported on other 
      specimens, such as paraffin-embedded tissue and unstained cytology slides. The 
      authors proposed that their technique would allow the pathologist and clinician 
      the flexibility to utilize previously stained fine-needle aspiration slides for 
      FISH evaluation.
FAU - Richmond, Jeffrey
AU  - Richmond J
AD  - Department of Pathology, Fletcher Allen Health Care/University of Vermont College 
      of Medicine, Burlington, Vermont 05401, USA.
FAU - Bryant, Ronald
AU  - Bryant R
FAU - Trotman, Winifred
AU  - Trotman W
FAU - Beatty, Barbara
AU  - Beatty B
FAU - Lunde, John
AU  - Lunde J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Biopsy, Fine-Needle
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics/pathology
MH  - Lymphoid Tissue/pathology
MH  - Lymphoma, Follicular/diagnosis/genetics/*pathology
MH  - Lymphoma, Mantle-Cell/diagnosis/genetics/pathology
MH  - Staining and Labeling
MH  - *Translocation, Genetic
EDAT- 2006/05/04 09:00
MHDA- 2006/08/02 09:00
CRDT- 2006/05/04 09:00
PHST- 2006/05/04 09:00 [pubmed]
PHST- 2006/08/02 09:00 [medline]
PHST- 2006/05/04 09:00 [entrez]
AID - 10.1002/cncr.21917 [doi]
PST - ppublish
SO  - Cancer. 2006 Jun 25;108(3):198-204. doi: 10.1002/cncr.21917.