PMID- 16671480
OWN - NLM
STAT- MEDLINE
DCOM- 20060612
LR  - 20190917
IS  - 0065-1419 (Print)
IS  - 0065-1419 (Linking)
VI  - 96
DP  - 2006
TI  - Post-traumatic application of brain-derived neurotrophic factor and glia-derived 
      neurotrophic factor on the rat spinal cord enhances neuroprotection and improves 
      motor function.
PG  - 329-34
AB  - We examined the potential efficacy of brain-derived neurotrophic factor (BDNF) 
      and glial-derived neurotrophic factor (GDNF) applied over traumatized spinal 
      cord, alone or in combination, for attenuating motor dysfunction, blood-spinal 
      cord barrier (BSCB) breakdown, edema formation, and cell injury in a rat model. 
      Under Equithesin anesthesia, spinal cord injury (SCI) was performed by making a 
      unilateral incision into the right dorsal horn of the T10-11 segment. The rats 
      were allowed to survive 5 hours after trauma. The BDNF or GDNF was applied (0.1 
      to 1 microg/10 microl in phosphate buffer saline) 30, 60, or 90 minutes after 
      SCI. Topical application of BDNF or GDNF 30 minutes after SCI in high 
      concentration (0.5 microg and 1 microg) significantly improved motor function and 
      reduced BSCB breakdown, edema formation, and cell injury at 5 hours. These 
      beneficial effects of neurotrophins were markedly absent when administered 
      separately either 60 or 90 minutes after injury. However, combined application of 
      BDNF and GDNF at 60 or 90 minutes after SCI resulted in a significant reduction 
      in motor dysfunction and spinal cord pathology. These novel observations suggest 
      that neurotrophins in combination have potential therapeutic value for the 
      treatment of SCI in clinical situations.
FAU - Sharma, H S
AU  - Sharma HS
AD  - Laboratory of Cerebrovascular Biology, Department of Surgical Sciences, 
      Anesthesiology and Intensive Care Medicine, University Hospital, Uppsala 
      University, Uppsala, Sweden. Sharma@surgsci.uu.se
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Acta Neurochir Suppl
JT  - Acta neurochirurgica. Supplement
JID - 100962752
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drug Combinations)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (glia-derived neurite-promoting factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Drug Combinations
MH  - Male
MH  - Nerve Tissue Proteins/*administration & dosage
MH  - Neuroprotective Agents/administration & dosage
MH  - Paraplegia/etiology/*physiopathology/*prevention & control
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord/drug effects/*physiopathology
MH  - Spinal Cord Injuries/complications/*drug therapy/*physiopathology
MH  - Treatment Outcome
EDAT- 2006/05/05 09:00
MHDA- 2006/06/13 09:00
CRDT- 2006/05/05 09:00
PHST- 2006/05/05 09:00 [pubmed]
PHST- 2006/06/13 09:00 [medline]
PHST- 2006/05/05 09:00 [entrez]
AID - 10.1007/3-211-30714-1_69 [doi]
PST - ppublish
SO  - Acta Neurochir Suppl. 2006;96:329-34. doi: 10.1007/3-211-30714-1_69.