PMID- 16671882
OWN - NLM
STAT- MEDLINE
DCOM- 20060719
LR  - 20091119
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 77
IP  - 5
DP  - 2006 May
TI  - Inhibitory effects of tumor necrosis factor-alpha on migration of human 
      periodontal ligament cells.
PG  - 883-90
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is associated with chronic 
      gingival inflammation and is suspected to influence periodontal destruction. 
      However, the exact roles of TNF-alpha in wound healing and periodontal tissue 
      regeneration are largely unknown. In the present study, we examined the effects 
      of TNF-alpha on migration and proliferation of human periodontal ligament (PDL) 
      cells. METHODS: PDL cells were cultured in the presence of TNF-alpha to determine 
      its effects on cellular migration and proliferation. The protein expression 
      profiles of alpha5 and beta1 integrin subunits and their related molecules, 
      paxillin and focal adhesion kinases (FAK), were investigated. Gene expression of 
      fibronectin also was assayed. Further, the activation of Rho-family small 
      guanosine triphosphate (GTP)-binding protein (RhoA) was evaluated using a 
      GTP-loading pull-down assay, and focal adhesion formation by PDL cells after 
      transfection with the expression vector of paxillin-fused green fluorescent 
      protein (GFP) also was observed with confocal microscopy. RESULTS: Cellular 
      migration was impaired by TNF-alpha and recovered following the addition of 
      anti-TNF-alpha antibodies. In contrast, PDL cell proliferation was not affected 
      by TNF-alpha. TNF-alpha upregulated the expression of the alpha5 and beta1 
      integrin subunits, whereas fibronectin was not overexpressed. Phosphorylation of 
      paxillin and FAK by PDL cells was induced, and RhoA activation also was induced. 
      Confocal microscopic analysis revealed that TNF-alpha induced focal adhesion and 
      stress fiber formation in all parts of the cells. CONCLUSION: Our results 
      suggested that TNF-alpha impairs cellular migration by enhancing cellular 
      adhesive ability following significant focal adhesion and stress fiber formation.
FAU - Takemura, Akane
AU  - Takemura A
AD  - Department of Oral Frontier Biology, Osaka University Graduate School of 
      Dentistry, Suita-Osaka, Japan.
FAU - Nakagawa, Ichiro
AU  - Nakagawa I
FAU - Kawai, Shinji
AU  - Kawai S
FAU - Inaba, Hiroaki
AU  - Inaba H
FAU - Kato, Takahiro
AU  - Kato T
FAU - Hamada, Shigeyuki
AU  - Hamada S
FAU - Amano, Atsuo
AU  - Amano A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Fibronectins)
RN  - 0 (Integrin alpha5beta1)
RN  - 0 (Paxillin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 124671-05-2 (RHOA protein, human)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Adolescent
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Female
MH  - Fibronectins/genetics
MH  - Focal Adhesion Protein-Tyrosine Kinases/drug effects/metabolism
MH  - Humans
MH  - Integrin alpha5beta1/drug effects/metabolism
MH  - Paxillin/drug effects/metabolism
MH  - Periodontal Ligament/*cytology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - rhoA GTP-Binding Protein/drug effects/metabolism
EDAT- 2006/05/05 09:00
MHDA- 2006/07/20 09:00
CRDT- 2006/05/05 09:00
PHST- 2006/05/05 09:00 [pubmed]
PHST- 2006/07/20 09:00 [medline]
PHST- 2006/05/05 09:00 [entrez]
AID - 10.1902/jop.2006.050192 [doi]
PST - ppublish
SO  - J Periodontol. 2006 May;77(5):883-90. doi: 10.1902/jop.2006.050192.