PMID- 16674193
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20221207
IS  - 1083-3668 (Print)
IS  - 1083-3668 (Linking)
VI  - 11
IP  - 2
DP  - 2006 Mar-Apr
TI  - Raman microspectrometry of laser-reshaped rabbit auricular cartilage: preliminary 
      study on laser-induced cartilage mineralization.
PG  - 024003
AB  - Laser-assisted cartilage reshaping (LACR) is a relatively novel technique 
      designed to noninvasively and permanently restructure cartilaginous tissue. It is 
      believed that heat-induced stress relaxation, in which a temperature-mediated 
      disruption of H2O binding is associated with conformational alterations in the 
      proteoglycan and collagen-rich matrix, constitutes the underlying mechanism of 
      LACR. Several reports have suggested that laser-mediated cartilage mineralization 
      may contribute to the permanent shape change of laser-reshaped cartilage. In an 
      effort to validate these results in the context of Er:glass LACR, we performed a 
      preliminary Raman microspectrometric study to characterize the crystal deposits 
      in laser-irradiated chondrocytes and extracellular matrix. For the first time, we 
      identified intracellular calcium sulfate deposits and extracellular calcium 
      phosphate (apatite) crystals in laser-reshaped rabbit auricular cartilage. 
      Calcium carbonate deposits are localized in both irradiated and nonirradiated 
      samples, suggesting that this mineral plays no role in conformational retention. 
      In our discussion, we elaborate on the possible molecular and cellular mechanisms 
      responsible for intra- and extracellular crystallization, and propose a novel 
      hypothesis on the formation of apatite, inasmuch as the biological function of 
      this mineral (providing structure and rigidity in bones and dental enamel) may be 
      extrapolated to the permanent shape change of laser-irradiated cartilage.
FAU - Heger, Michal
AU  - Heger M
AD  - University of Amsterdam, Academic Medical Center, Laser Center, Amsterdam, The 
      Netherlands. M.Heger@amc.uva.nl
FAU - Mordon, Serge
AU  - Mordon S
FAU - Leroy, Gerard
AU  - Leroy G
FAU - Fleurisse, Laurence
AU  - Fleurisse L
FAU - Creusy, Colette
AU  - Creusy C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
RN  - 0 (Minerals)
SB  - IM
MH  - Animals
MH  - Ear Cartilage/*metabolism/*radiation effects
MH  - Female
MH  - Laser Therapy/*methods
MH  - Minerals/*analysis
MH  - Pilot Projects
MH  - Rabbits
MH  - Plastic Surgery Procedures/*methods
MH  - Spectrum Analysis, Raman/*methods
MH  - Treatment Outcome
EDAT- 2006/05/06 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/05/06 09:00
PHST- 2006/05/06 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/05/06 09:00 [entrez]
AID - 10.1117/1.2187420 [doi]
PST - ppublish
SO  - J Biomed Opt. 2006 Mar-Apr;11(2):024003. doi: 10.1117/1.2187420.