PMID- 16674601
OWN - NLM
STAT- MEDLINE
DCOM- 20060731
LR  - 20141120
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 55
IP  - 6
DP  - 2006 Jun
TI  - Effect of toll-like receptor (TLR) agonists on TLR and microbicide expression in 
      uterine and vaginal tissues of the mouse.
PG  - 434-46
AB  - PROBLEM: Epithelial cells lining the uterine lumen are the first line of defense 
      against pathogenic microbes. The objective of this study was to examine the 
      expression of Toll-like receptors (TLRs), defensins and secretory leukocyte 
      protease inhibitor (SLPI) in the mouse uterus and vagina and in primary uterine 
      epithelial cells and to determine whether TLR agonists induce TLR and defensin 
      expression. METHOD OF STUDY: The mRNA expression of alpha- and beta-defensins 
      (AD1, 2 and 5 and BD1, 2 and 4) and SLPI was examined by real-time reverse 
      transcriptase-polymerase chain reaction (RT-PCR) along with the secretion of 
      macrophage chemotactic protein-1 (MCP-1), measured by enzyme-linked immunosorbent 
      assay. RESULTS: Expression of TLR1-9 as well as beta-defensins 1, 2 and 4 and 
      SLPI by uterine and vaginal tissues was demonstrated by RT-PCR. beta-Defensins 
      and SLPI expression was greater in the vagina than in the uterus. Comparison of 
      fresh and polarized uterine epithelial cells indicated that TLR2-6 expression was 
      unaffected by culture. Incubation of polarized epithelial cells with TLR agonists 
      [lipopolysaccharide (LPS), Pam3Cys, Poly (I:C) or PGN] induced TLR5 and TLR9 
      expression but had no effect on TLR4, defensins or SLPI. Furthermore, exposure to 
      LPS, Pam3Cys, Poly (I:C) or PGN, induced MCP-1 secretion by polarized epithelial 
      cells in culture. CONCLUSION: These results indicate that the uterus and vagina 
      as well as uterine epithelial cells are responsive to bacterial and viral 
      pathogens. Not only do epithelial cells respond to TLR agonists by releasing 
      MCP-1, which mediates inflammatory responses, but they also influence the 
      expression of selected TLR genes to further enhance innate immune protection.
FAU - Soboll, Gisela
AU  - Soboll G
AD  - Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
FAU - Schaefer, Todd M
AU  - Schaefer TM
FAU - Wira, Charles R
AU  - Wira CR
LA  - eng
GR  - AI-13541/AI/NIAID NIH HHS/United States
GR  - AI-33478/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Defensins)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Secretory Leukocyte Peptidase Inhibitor)
RN  - 0 (Slpi protein, mouse)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Defensins/genetics
MH  - Epithelial Cells/drug effects/immunology/metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Immunity, Innate
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Proteinase Inhibitory Proteins, Secretory
MH  - Proteins/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Secretory Leukocyte Peptidase Inhibitor
MH  - Toll-Like Receptors/*agonists/genetics/*metabolism
MH  - Uterus/*drug effects/*immunology/metabolism
MH  - Vagina/*drug effects/*immunology/metabolism
EDAT- 2006/05/06 09:00
MHDA- 2006/08/01 09:00
CRDT- 2006/05/06 09:00
PHST- 2006/05/06 09:00 [pubmed]
PHST- 2006/08/01 09:00 [medline]
PHST- 2006/05/06 09:00 [entrez]
AID - AJI381 [pii]
AID - 10.1111/j.1600-0897.2006.00381.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2006 Jun;55(6):434-46. doi: 
      10.1111/j.1600-0897.2006.00381.x.