PMID- 16674682
OWN - NLM
STAT- MEDLINE
DCOM- 20060728
LR  - 20131121
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 114 Suppl 1
DP  - 2006 May
TI  - Vitamin D and tissue non-specific alkaline phosphatase in dental cells.
PG  - 178-82; discussion 201-2, 381
AB  - Dental epithelium comprises different cell populations, including ameloblasts and 
      stratum intermedium cells. Ameloblasts are vitamin D targets, and at least five 
      proteins undergo specific modulation of their expression following the addition 
      of 1alpha,25(OH)2 vitamin D3[1alpha,25(OH)2D3]. Stratum intermedium cells have 
      not been studied in any great detail regarding vitamin D impact. Interestingly, 
      in these cells, the tissue non-specific alkaline phosphatase (TNAP) is 
      overexpressed. On the other hand, TNAP is a reliable bone marker of vitamin D 
      action, similar to calbindins in kidney and intestine, previously used for 
      studies of vitamin D activity in ameloblasts. Here, TNAP expression and activity 
      were investigated in vivo in the microdissected epithelium and mesenchyme of 
      mandible incisors. Physiological doses of 1alpha,25(OH)2D3 injected in control 
      rats failed to modify TNAP activity in both dental epithelium and mesenchyme. No 
      significant differences were observed in the steady-state levels of TNAP mRNAs of 
      dental tissues from wild-type and vitamin D nuclear receptor (VDRnuc)-deficient 
      mice of the same litters. These data suggest that, in contrast to ameloblasts, 
      stratum intermedium cells are not sensitive to 1alpha,25(OH)2D3. An explanation 
      for such a responsiveness of stratum intermedium cells to 1alpha,25(OH)2D3 is 
      proposed based on the respective expressions of both vitamin D receptors (VDRnuc 
      and 1,25D3-[MARRS]) and the Dlx2 homeobox gene.
FAU - Lezot, Frederic
AU  - Lezot F
AD  - INSERM U714, Paris F-75006 France.
FAU - Descroix, Vianney
AU  - Descroix V
FAU - Hotton, Dominique
AU  - Hotton D
FAU - Mauro, Nicole
AU  - Mauro N
FAU - Kato, Shigeaki
AU  - Kato S
FAU - Berdal, Ariane
AU  - Berdal A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Amelogenin)
RN  - 0 (Amelx protein, mouse)
RN  - 0 (Amelx protein, rat)
RN  - 0 (Dental Enamel Proteins)
RN  - 0 (Distal-less homeobox proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Transcription Factors)
RN  - 0 (Vitamins)
RN  - 1406-16-2 (Vitamin D)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Alkaline Phosphatase/*drug effects
MH  - Ameloblasts/drug effects
MH  - Amelogenesis/genetics
MH  - Amelogenin
MH  - Animals
MH  - Calcitriol/pharmacology
MH  - Dental Enamel Proteins/drug effects
MH  - Enamel Organ/*drug effects
MH  - Epithelium/drug effects
MH  - Homeodomain Proteins/genetics
MH  - Incisor
MH  - Mesoderm/drug effects
MH  - Mice
MH  - Mice, Transgenic
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Calcitriol/deficiency/genetics
MH  - Tooth Germ/*drug effects
MH  - Transcription Factors/genetics
MH  - Vitamin D/*pharmacology
MH  - Vitamins/*pharmacology
EDAT- 2006/05/06 09:00
MHDA- 2006/07/29 09:00
CRDT- 2006/05/06 09:00
PHST- 2006/05/06 09:00 [pubmed]
PHST- 2006/07/29 09:00 [medline]
PHST- 2006/05/06 09:00 [entrez]
AID - EOS338 [pii]
AID - 10.1111/j.1600-0722.2006.00338.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2006 May;114 Suppl 1:178-82; discussion 201-2, 381. doi: 
      10.1111/j.1600-0722.2006.00338.x.