PMID- 16675561
OWN - NLM
STAT- MEDLINE
DCOM- 20060821
LR  - 20201226
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 12
IP  - 9
DP  - 2006 May 1
TI  - Mechanisms and effects of loss of human leukocyte antigen class II expression in 
      immune-privileged site-associated B-cell lymphoma.
PG  - 2698-705
AB  - PURPOSE AND EXPERIMENTAL DESIGN: Loss of human leukocyte antigen (HLA) expression 
      on tumor cells is frequent in diffuse large B-cell lymphoma (DLBCL) arising in 
      immune-privileged sites, such as the testis and central nervous system, and is 
      associated with small homozygous deletions of HLA-DQ/HLA-DR and larger hemizygous 
      deletions of the MHC region. To better understand the significance of 
      down-regulation of HLA class II expression in relation to the homozygous and 
      hemizygous deletions, we analyzed global gene expression patterns in a series of 
      26 testicular DLBCL after characterization of these deletions. RESULTS: Low 
      levels of HLA-DR mRNA in whole testicular DLBCL samples were associated with a 
      strong down-regulation of numerous immune-related genes specific for T cells, 
      macrophages, antigen presentation and processing, lymphocyte activation, 
      chemokines and chemokine receptors, and the complement system. The number of CD3+ 
      tumor-infiltrating T cells was also significantly lower in low expressors of 
      HLA-DR mRNA. Interestingly, hemizygous and homozygous deletions in the MHC region 
      did not have any additional effect on global gene expression. CONCLUSION: In 
      conclusion, we found that loss of HLA class II mRNA expression in testicular 
      DLBCL is associated with a significant change in global gene expression patterns. 
      This effect is independent of the mechanism causing the down-regulation of HLA 
      class II genes in the lymphoma cells.
FAU - Booman, Marije
AU  - Booman M
AD  - Department of Pathology and Laboratory Medicine, University Medical Center 
      Groningen, Groningen, Netherlands. m.booman@path.umcg.nl
FAU - Douwes, Jenny
AU  - Douwes J
FAU - Glas, Annuska M
AU  - Glas AM
FAU - Riemersma, Sietske A
AU  - Riemersma SA
FAU - Jordanova, Ekaterina S
AU  - Jordanova ES
FAU - Kok, Klaas
AU  - Kok K
FAU - Rosenwald, Andreas
AU  - Rosenwald A
FAU - de Jong, Daphne
AU  - de Jong D
FAU - Schuuring, Ed
AU  - Schuuring E
FAU - Kluin, Philip M
AU  - Kluin PM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (HLA-D Antigens)
SB  - IM
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 6
MH  - Gene Expression Regulation, Neoplastic/*immunology
MH  - HLA-D Antigens/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymph Nodes/immunology/pathology
MH  - Lymphoma, B-Cell/genetics/*immunology/pathology
MH  - Lymphoma, Large-Cell, Immunoblastic/genetics/immunology/pathology
MH  - Major Histocompatibility Complex
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Sequence Deletion
MH  - Telomere/genetics
MH  - Testis/immunology
EDAT- 2006/05/06 09:00
MHDA- 2006/08/22 09:00
CRDT- 2006/05/06 09:00
PHST- 2006/05/06 09:00 [pubmed]
PHST- 2006/08/22 09:00 [medline]
PHST- 2006/05/06 09:00 [entrez]
AID - 12/9/2698 [pii]
AID - 10.1158/1078-0432.CCR-05-2617 [doi]
PST - ppublish
SO  - Clin Cancer Res. 2006 May 1;12(9):2698-705. doi: 10.1158/1078-0432.CCR-05-2617.