PMID- 16676162
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20131121
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 81
IP  - 1
DP  - 2007 Jan
TI  - Protection effect of piper betel leaf extract against carbon 
      tetrachloride-induced liver fibrosis in rats.
PG  - 45-55
AB  - Piper betel leaves (PBL) are used in Chinese folk medicine for the treatment of 
      various disorders. PBL has the biological capabilities of detoxication, 
      antioxidation, and antimutation. In this study, we evaluated the antihepatotoxic 
      effect of PBL extract on the carbon tetrachloride (CCl(4))-induced liver injury 
      in a rat model. Fibrosis and hepatic damage, as reveled by histology and the 
      activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) 
      were induced in rats by an administration of CCl(4) (8%, 1 ml/kg body weight) 
      thrice a week for 4 weeks. PBL extract significantly inhibited the elevated AST 
      and ALT activities caused by CCl(4) intoxication. It also attenuated total 
      glutathione S-transferase (GST) activity and GST alpha isoform activity, and on 
      the other hand, enhanced superoxide dismutase (SOD) and catalase (CAT) 
      activities. The histological examination showed the PBL extract protected liver 
      from the damage induced by CCl(4) by decreasing alpha-smooth muscle actin 
      (alpha-sma) expression, inducing active matrix metalloproteinase-2 (MMP2) 
      expression though Ras/Erk pathway, and inhibiting TIMP2 level that consequently 
      attenuated the fibrosis of liver. The data of this study support a 
      chemopreventive potential of PBL against liver fibrosis.
FAU - Young, Shun-Chieh
AU  - Young SC
AD  - Institute of Biochemistry and Biotechnology, Chung Shan Medical University, No. 
      110, Section 2, Chien Kauo N. Road, Taichung 402, Taiwan.
FAU - Wang, Chau-Jong
AU  - Wang CJ
FAU - Lin, Jing-Jing
AU  - Lin JJ
FAU - Peng, Pei-Ling
AU  - Peng PL
FAU - Hsu, Jui-Ling
AU  - Hsu JL
FAU - Chou, Fen-Pi
AU  - Chou FP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060505
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Actins)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (smooth muscle actin, rat)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Actins/metabolism
MH  - Alanine Transaminase/metabolism
MH  - Animals
MH  - Aspartate Aminotransferases/metabolism
MH  - Blotting, Western
MH  - Body Weight/drug effects
MH  - Carbon Tetrachloride/administration & dosage/*toxicity
MH  - Catalase/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/chemistry/isolation & purification/*therapeutic use
MH  - Glutathione Transferase/metabolism
MH  - Hydroxyl Radical/metabolism
MH  - Injections, Intraperitoneal
MH  - Liver/drug effects/metabolism/pathology
MH  - Liver Cirrhosis, Experimental/chemically induced/drug therapy/*prevention & 
      control
MH  - Male
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - Phytotherapy
MH  - Piper betle/*chemistry
MH  - Plant Leaves/*chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/metabolism
EDAT- 2006/05/06 09:00
MHDA- 2007/10/25 09:00
CRDT- 2006/05/06 09:00
PHST- 2006/01/13 00:00 [received]
PHST- 2006/03/27 00:00 [accepted]
PHST- 2006/05/06 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2006/05/06 09:00 [entrez]
AID - 10.1007/s00204-006-0106-0 [doi]
PST - ppublish
SO  - Arch Toxicol. 2007 Jan;81(1):45-55. doi: 10.1007/s00204-006-0106-0. Epub 2006 May 
      5.