PMID- 1667624
OWN - NLM
STAT- MEDLINE
DCOM- 19920619
LR  - 20190705
IS  - 0009-2363 (Print)
IS  - 0009-2363 (Linking)
VI  - 39
IP  - 12
DP  - 1991 Dec
TI  - Low molecular weight heparin enhances prostacyclin production by cultured human 
      endothelial cells.
PG  - 3368-9
AB  - Confluent cultures of vascular endothelial cells derived from the human umbilical 
      vein were incubated in a serum-free medium in the presence of low molecular 
      weight heparin (LMWH) with molecular weights of 4000-6000 dalton (Da), or of 
      unfractionated heparin (UFH) with average molecular weight 12,000 Da, and 
      prostacyclin production was determined by radioimmunoassay for 
      6-keto-prostaglandin F1 alpha, the stable metabolite of prostacyclin. LMWH at 1 
      U/ml as anti-factor Xa activity significantly increased prostacyclin production 
      after 6h or longer; however, UFH at 1 USP U/ml did not induce such a significant 
      change. The LMWH-induced increase in prostacyclin production occurred at 0.1 U/ml 
      and above after 6 h of treatment. Since prostacyclin is both a potent inhibitor 
      of platelet aggregation and a vasodilator, it was suggested that the increased 
      endothelial cell prostacyclin production induced by LMWH may be a component of 
      the anticoagulant activity of the drug.
FAU - Kaji, T
AU  - Kaji T
AD  - Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku 
      University, Kanazawa, Japan.
FAU - Yamamoto, C
AU  - Yamamoto C
FAU - Sakamoto, M
AU  - Sakamoto M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - DCR9Z582X0 (Epoprostenol)
SB  - IM
MH  - Cells, Cultured
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Epoprostenol/*biosynthesis
MH  - Heparin, Low-Molecular-Weight/*pharmacology
MH  - Humans
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
AID - 10.1248/cpb.39.3368 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 1991 Dec;39(12):3368-9. doi: 10.1248/cpb.39.3368.