PMID- 16676330
OWN - NLM
STAT- MEDLINE
DCOM- 20060919
LR  - 20211020
IS  - 0360-4012 (Print)
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 84
IP  - 2
DP  - 2006 Aug 1
TI  - Decreased NR1 phosphorylation and decreased NMDAR function in hibernating Arctic 
      ground squirrels.
PG  - 291-8
AB  - Heterothermic mammals such as ground squirrels tolerate ischemia and 
      N-methyl-D-aspartate (NMDA) better than homeothermic mammals such as rats both in 
      vivo and in vitro, and this tolerance is enhanced in the hibernating state. 
      However, the cellular mechanisms underlying this tolerance remain unclear. NMDA 
      receptors (NMDAR) play a key role in excitotoxicity. The purpose of the current 
      study was therefore to test the hypothesis that NMDAR are down-regulated in 
      hibernating Arctic ground squirrels (hAGS; Spermophilus parryii). To address this 
      hypothesis, we used Western blot analysis to investigate NMDAR phosphorylation, 
      an activator of NMDAR function, and internalization in naive hippocampal tissue 
      from hAGS, interbout euthermic AGS (ibeAGS), and rats. Furthermore, we used 
      fura-2 calcium imaging to examine NMDAR function in cultured hippocampal slices 
      from hAGS, ibeAGS, and rats. We report that phosphorylation of the NMDAR1 (NR1) 
      subunit is decreased in hippocampal tissue from hAGS and that the NMDAR component 
      of Glu-induced increase in [Ca(2+)](i) is decreased in hippocampal slices from 
      hAGS. Moreover, the fraction of NR1 in the functional membrane pool in AGS is 
      less than that in rats.
FAU - Zhao, Huiwen W
AU  - Zhao HW
AD  - Alaskan Basic Neuroscience Program, Institute of Arctic Biology, University of 
      Alaska Fairbanks, 99775-7000, USA.
FAU - Ross, Austin P
AU  - Ross AP
FAU - Christian, Sherri L
AU  - Christian SL
FAU - Buchholz, John N
AU  - Buchholz JN
FAU - Drew, Kelly L
AU  - Drew KL
LA  - eng
GR  - U54 NS041069/NS/NINDS NIH HHS/United States
GR  - NIH 41069/PHS HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain Chemistry
MH  - Calcium/metabolism
MH  - Down-Regulation
MH  - Glutamic Acid/metabolism
MH  - Hibernation/*physiology
MH  - Hippocampus
MH  - Imaging, Three-Dimensional
MH  - Organ Culture Techniques
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Sciuridae/*physiology
PMC - PMC3796386
MID - NIHMS451080
EDAT- 2006/05/06 09:00
MHDA- 2006/09/20 09:00
PMCR- 2013/10/14
CRDT- 2006/05/06 09:00
PHST- 2006/05/06 09:00 [pubmed]
PHST- 2006/09/20 09:00 [medline]
PHST- 2006/05/06 09:00 [entrez]
PHST- 2013/10/14 00:00 [pmc-release]
AID - 10.1002/jnr.20893 [doi]
PST - ppublish
SO  - J Neurosci Res. 2006 Aug 1;84(2):291-8. doi: 10.1002/jnr.20893.