PMID- 16677760
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20211203
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 401
IP  - 1-2
DP  - 2006 Jun 19
TI  - A role for the mTOR pathway in surface expression of AMPA receptors.
PG  - 35-9
AB  - Delivery of alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionate receptors 
      (AMPARs) to the synapse is a critical factor controlling synaptic strength. It is 
      now established that blockade of synaptic activity increases the surface 
      expression of AMPARs. Factors modulating the delivery, insertion and expression 
      of AMPARs are not completely known. Using immunohistochemical techniques, we 
      first confirmed rapamycin-mediated inhibition of the mammalian target of 
      rapamycin (mTOR) pathway in cortical neuronal culture. We then demonstrated that 
      acute AMPAR activity blockade increased the synaptic expression of GluR2/3 
      subunits and rapamycin significantly reduced this expression. Our results suggest 
      a role for the mTOR pathway in surface expression of AMPA receptors on cortical 
      neurons.
FAU - Wang, Yanling
AU  - Wang Y
AD  - Epilepsy Research Laboratory in the Department of Neurological Surgery and PIBS 
      Graduate Program in Neuroscience, University of California, Box 0520, 513 
      Parnassus Ave., San Francisco, CA 94143, USA.
FAU - Barbaro, Michael F
AU  - Barbaro MF
FAU - Baraban, Scott C
AU  - Baraban SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060503
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (glutamate receptor ionotropic, AMPA 3)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - P6W5IXV8V9 (glutamate receptor ionotropic, AMPA 2)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/metabolism
MH  - Dendrites/metabolism/ultrastructure
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Glutamic Acid/*metabolism
MH  - Immunosuppressive Agents/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neurons/cytology/*metabolism
MH  - Protein Kinases/genetics/*metabolism
MH  - Receptor Aggregation/*genetics
MH  - Receptors, AMPA/antagonists & inhibitors/*metabolism
MH  - Receptors, Cell Surface/antagonists & inhibitors/metabolism
MH  - Sirolimus/pharmacology
MH  - Synaptic Transmission/*genetics
MH  - TOR Serine-Threonine Kinases
EDAT- 2006/05/09 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/05/09 09:00
PHST- 2005/11/15 00:00 [received]
PHST- 2006/03/01 00:00 [revised]
PHST- 2006/03/01 00:00 [accepted]
PHST- 2006/05/09 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/05/09 09:00 [entrez]
AID - S0304-3940(06)00252-7 [pii]
AID - 10.1016/j.neulet.2006.03.011 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 Jun 19;401(1-2):35-9. doi: 10.1016/j.neulet.2006.03.011. Epub 
      2006 May 3.