PMID- 16677851
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 606
IP  - 1-2
DP  - 2006 Jul 14
TI  - Antioxidant and antimutagenic activity of mannan neoglycoconjugates: mannan-human 
      serum albumin and mannan-penicillin G acylase.
PG  - 72-9
AB  - The antioxidant and antimutagenic activity of the yeast cell-wall mannan and 
      mannan conjugates--in particular the mannan of Saccharomyces cerevisiae (M-S.c.) 
      and conjugates of mannan S. cerevisiae with human serum albumin (M-HSA1, M-HSA2) 
      and the microbial enzyme penicillin G acylase (M-PGA)--were evaluated in vitro in 
      the unicellular flagellate Euglena gracilis exposed to the genotoxic agents 
      ofloxacin and acridine orange (AO). M-S.c., M-HSA1, M-HSA2 and M-PGA show a 
      statistically significant, concentration-dependent protective antigenotoxic 
      activity against both compounds. M-PGA was the most efficient inhibitor of 
      ofloxacin- and AO-induced chloroplast DNA damage, whereas M-HSA2 and M-HSA1 were 
      less effective and M-S.c. had the lowest antigenotoxic activity. It is suggested 
      that different mechanisms may be involved in their protective effect--antioxidant 
      activity in the case of ofloxacin-induced DNA damage and direct adsorption of AO 
      on mannan conjugates as possible mechanisms of protection, based on 
      spectrophotometric measurements. The important characteristics of yeast cell-wall 
      mannans and mannan conjugates, such as their high water solubility, their broad 
      spectrum of biological activity, low toxicity, stability and their antimutagenic 
      effects via different modes of action, appear to be promising features for their 
      practical application as antioxidants and antimutagenic agents.
FAU - Krizkova, Livia
AU  - Krizkova L
AD  - Institute of Cell Biology, Comenius University, Faculty of Natural Sciences, 
      Mlynska dolina, 842 15 Bratislava, Slovak Republic. krizkova@fns.uniba.sk
FAU - Zitnanova, Ingrid
AU  - Zitnanova I
FAU - Mislovicova, Danica
AU  - Mislovicova D
FAU - Masarova, Jana
AU  - Masarova J
FAU - Sasinkova, Vlasta
AU  - Sasinkova V
FAU - Durackova, Zdenka
AU  - Durackova Z
FAU - Krajcovic, Juraj
AU  - Krajcovic J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060504
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Albumins)
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Mannans)
RN  - 0 (Mutagens)
RN  - 0 (Serum Albumin)
RN  - 0 (mannosyl-neoglycoalbumin)
RN  - A4P49JAZ9H (Ofloxacin)
RN  - EC 3.5.1.11 (Penicillin Amidase)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Albumins/chemistry/*metabolism
MH  - Animals
MH  - Antimutagenic Agents/chemistry/*metabolism
MH  - Antioxidants/chemistry/*metabolism
MH  - Euglena gracilis/drug effects
MH  - Humans
MH  - Mannans/chemistry/*metabolism
MH  - Mutagenicity Tests
MH  - Mutagens/*pharmacology
MH  - Ofloxacin/pharmacology
MH  - Penicillin Amidase/chemistry/*metabolism
MH  - Saccharomyces cerevisiae/chemistry/cytology/drug effects/metabolism
MH  - Serum Albumin/chemistry/*metabolism
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2006/05/09 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/05/09 09:00
PHST- 2005/07/20 00:00 [received]
PHST- 2005/12/15 00:00 [revised]
PHST- 2006/03/13 00:00 [accepted]
PHST- 2006/05/09 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/05/09 09:00 [entrez]
AID - S1383-5718(06)00078-7 [pii]
AID - 10.1016/j.mrgentox.2006.03.003 [doi]
PST - ppublish
SO  - Mutat Res. 2006 Jul 14;606(1-2):72-9. doi: 10.1016/j.mrgentox.2006.03.003. Epub 
      2006 May 4.