PMID- 16679561
OWN - NLM
STAT- MEDLINE
DCOM- 20060713
LR  - 20171116
IS  - 1085-9195 (Print)
IS  - 1085-9195 (Linking)
VI  - 45
IP  - 1
DP  - 2006
TI  - Biophysical studies on the differentiation of human CD14+ monocytes into 
      dendritic cells.
PG  - 19-30
AB  - Dendritic cells (DCs), which are the most efficient antigen-presenting cells 
      (APCs) currently known, can be derived from CD14+ monocytes (DC predecessor 
      cells) in vitro. Immature DCs actively take up antigens and pathogens, generate 
      major histocompatability complex-peptide complexes, and migrate from the sites of 
      antigen acquisition to secondary lymphoid organs to become mature dendritic cells 
      that interact with and stimulate T-lymphocytes. During this process, the cells 
      must undergo deformation to translocate through several barriers, including the 
      basement membrane and interstitial connective tissue in the blood vessel wall. To 
      further understand the mechanisms of the activation of immunological responses 
      and the migration from peripheral tissue to secondary lymphoid organs, we have 
      applied biophysical and microrheological methods to study the development 
      processes of DCs in vitro. The results showed that membrane fluidity, osmotic 
      fragility, membrane viscoelastic properties, infrared spectroscopy, and 
      cytoskeleton organization of DCs exhibit significant differences in different 
      developmental stages.
FAU - Zeng, Zhu
AU  - Zeng Z
AD  - Department of Biophysics, Health Science Center, Peking University, Beijing, 
      People's Republic of China.
FAU - Liu, Xiao
AU  - Liu X
FAU - Jiang, Yuhui
AU  - Jiang Y
FAU - Wang, Guotao
AU  - Wang G
FAU - Zhan, Jun
AU  - Zhan J
FAU - Guo, Jun
AU  - Guo J
FAU - Yao, Weijuan
AU  - Yao W
FAU - Sun, Dagong
AU  - Sun D
FAU - Ka, Weibo
AU  - Ka W
FAU - Tang, Yan
AU  - Tang Y
FAU - Tang, Junming
AU  - Tang J
FAU - Wen, Zongyao
AU  - Wen Z
FAU - Chien, Shu
AU  - Chien S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Lipopolysaccharide Receptors)
SB  - IM
MH  - Biophysical Phenomena
MH  - *Biophysics
MH  - *Cell Differentiation
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lipopolysaccharide Receptors/*immunology
MH  - Models, Biological
MH  - Monocytes/*immunology
MH  - Rheology
MH  - Viscosity
EDAT- 2006/05/09 09:00
MHDA- 2006/07/14 09:00
CRDT- 2006/05/09 09:00
PHST- 1999/11/30 00:00 [received]
PHST- 1999/11/30 00:00 [revised]
PHST- 1999/11/30 00:00 [accepted]
PHST- 2006/05/09 09:00 [pubmed]
PHST- 2006/07/14 09:00 [medline]
PHST- 2006/05/09 09:00 [entrez]
AID - CBB:45:1:19 [pii]
AID - 10.1385/CBB:45:1:19 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2006;45(1):19-30. doi: 10.1385/CBB:45:1:19.