PMID- 16679564
OWN - NLM
STAT- MEDLINE
DCOM- 20060713
LR  - 20211020
IS  - 1085-9195 (Print)
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 45
IP  - 1
DP  - 2006
TI  - Single-step multicolor fluorescence in situ hybridization using semiconductor 
      quantum dot-DNA conjugates.
PG  - 59-70
AB  - We report a rapid method for the direct multicolor imaging of multiple subnuclear 
      genetic sequences using novel quantum dot-based fluorescence in situ 
      hybridization (FISH) probes (QD-FISH). Short DNA oligonucleotides were attached 
      on QDs and used in a single hybridization/detection step of target sites in situ. 
      QD-FISH probes penetrate both intact interphase nuclei and metaphase chromosomes 
      and showed good targeting of dense chromatin domains with minimal steric 
      hindrances. We further demonstrated that QD's broad absorption spectra allowed 
      different colored probes specific for distinct subnuclear genetic sequences to be 
      simultaneously excited with a single excitation wavelength and imaged free of 
      chromatic aberrations in a single exposure. Thus, these results demonstrate that 
      QD-FISH probes are very effective in multicolor FISH applications. This work also 
      documents new possibilities of using QD-FISH probes detection down to the single 
      molecule level.
FAU - Bentolila, Laurent A
AU  - Bentolila LA
AD  - Department of Chemistry and Biochemistry, David Geffen School of Medicine, 
      University of California at Los Angeles, Los Angeles, CA 90095-1569, USA. 
      lbento@chem.ucla.edu
FAU - Weiss, Shimon
AU  - Weiss S
LA  - eng
GR  - R01 EB000312/EB/NIBIB NIH HHS/United States
GR  - R01 EB000312-04/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Heterochromatin)
RN  - 0 (Oligonucleotides)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Biotinylation
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Centromere/chemistry
MH  - Color
MH  - DNA/*chemistry
MH  - HeLa Cells
MH  - Heterochromatin/chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mast Cells/cytology/*metabolism
MH  - Mice
MH  - Microscopy, Confocal
MH  - Models, Chemical
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotides/chemical synthesis/chemistry
MH  - Protein Structure, Tertiary
MH  - *Quantum Dots
PMC - PMC3084375
MID - NIHMS285483
EDAT- 2006/05/09 09:00
MHDA- 2006/07/14 09:00
PMCR- 2011/04/29
CRDT- 2006/05/09 09:00
PHST- 1999/11/30 00:00 [received]
PHST- 1999/11/30 00:00 [revised]
PHST- 1999/11/30 00:00 [accepted]
PHST- 2006/05/09 09:00 [pubmed]
PHST- 2006/07/14 09:00 [medline]
PHST- 2006/05/09 09:00 [entrez]
PHST- 2011/04/29 00:00 [pmc-release]
AID - CBB:45:1:59 [pii]
AID - 10.1385/CBB:45:1:59 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2006;45(1):59-70. doi: 10.1385/CBB:45:1:59.