PMID- 16680689
OWN - NLM
STAT- MEDLINE
DCOM- 20070213
LR  - 20200930
IS  - 1552-4973 (Print)
IS  - 1552-4973 (Linking)
VI  - 80
IP  - 1
DP  - 2007 Jan
TI  - Removal of noncollagenous components affects dentin bonding.
PG  - 86-91
AB  - The structural integrity of fibrillar type I collagen is critical for effective 
      dentin bonding. Since most noncollagenous matrix components in dentin are closely 
      associated with collagen, we hypothesized that they may also contribute to dentin 
      bonding. To test this hypothesis, bovine dentin was acid-etched, treated with 
      chondroitinase ABC (C-ABC), endo-beta-galactosidase (Endo-beta), or trypsin. 
      Controls were prepared in the same manner but without the enzymes. All control 
      and experimental specimens were then bonded with One-Step. Bond strength data 
      were analyzed by one-way ANOVA and Fisher's PLSD test (p < 0.05). When dentin was 
      treated with C-ABC or trypsin, bond strengths significantly decreased for the 
      rewetted groups (p < 0.05). The treatment with Endo-beta showed no effects on 
      bond strengths (p > 0.05). When the treated dentin surfaces were observed under 
      SEM, the C-ABC and trypsin treated groups revealed significant loss of collagen 
      fibril architecture. The results indicate that chondroitin sulfate 
      glycosaminoglycans and trypsin-digestible noncollagenous proteins play roles in 
      maintaining the open dimensions of the collagen fibril scaffold, which is 
      essential for optimal dentin bonding.
CI  - 2006 Wiley Periodicals, Inc.
FAU - Pereira, P N R
AU  - Pereira PN
AD  - Department of Operative Dentistry, University of North Carolina, Chapel Hill, 
      North Carolina, USA. patricia_pereira@dentistry.unc.edu
FAU - Bedran-de-Castro, A K B
AU  - Bedran-de-Castro AK
FAU - Duarte, W R
AU  - Duarte WR
FAU - Yamauchi, M
AU  - Yamauchi M
LA  - eng
GR  - DE016116/DE/NIDCR NIH HHS/United States
GR  - DE10489/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Clearfil AP-X)
RN  - 0 (Collagen Type I)
RN  - 0 (Methacrylates)
RN  - EC 3.- (Hydrolases)
SB  - IM
MH  - Acid Etching, Dental
MH  - Animals
MH  - Cattle
MH  - Collagen Type I/*chemistry
MH  - *Dental Bonding
MH  - Dentin/*chemistry/ultrastructure
MH  - Hydrolases/chemistry
MH  - Incisor/*chemistry/ultrastructure
MH  - Materials Testing
MH  - Methacrylates/*chemistry
MH  - Microscopy, Electron, Scanning
MH  - Surface Properties
EDAT- 2006/05/09 09:00
MHDA- 2007/02/14 09:00
CRDT- 2006/05/09 09:00
PHST- 2006/05/09 09:00 [pubmed]
PHST- 2007/02/14 09:00 [medline]
PHST- 2006/05/09 09:00 [entrez]
AID - 10.1002/jbm.b.30572 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2007 Jan;80(1):86-91. doi: 
      10.1002/jbm.b.30572.