PMID- 1668104
OWN - NLM
STAT- MEDLINE
DCOM- 19920730
LR  - 20190814
IS  - 0024-4201 (Print)
IS  - 0024-4201 (Linking)
VI  - 26
IP  - 12
DP  - 1991 Dec
TI  - Platelet-activating factor may participate in signal transduction processes in 
      rabbit leukocytes.
PG  - 1044-9
AB  - The bacterial chemotactic peptide, formyl-methionyl-leucyl-phenylalanine (fMLP), 
      induces the generation of platelet-activating factor (PAF), the mobilization of 
      arachidonic acid and generation of superoxide anion (O2-) in rabbit 
      polymorphonuclear leukocytes (PMNs). The PAF receptor antagonists, WEB 2086 
      (10-100 microM) and CV 6209 (1-10 microM), reduced the mobilization of 
      arachidonic acid and the O2- generation in response to fMLP but not that in 
      response to A23187. Pretreatment of PMNs with the phospholipase A2 inhibitor, 
      chloroquine, or the serine protease inhibitor, tosyl-phenylalanine chloromethyl 
      ketone, reduced the fMLP-stimulated generation of PAF and also reduced the 
      generation of O2-. The respiratory burst induced by a submaximal concentration of 
      phorbol myristate acetate was not affected by these compounds. These data are 
      consistent with the suggestion that endogenous PAF may contribute to the signal 
      transduction cascade initiated by fMLP.
FAU - Stewart, A G
AU  - Stewart AG
AD  - Department of Physiology, University of Melbourne, Victoria, Australia.
FAU - Harris, T
AU  - Harris T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lipids
JT  - Lipids
JID - 0060450
RN  - 0 (Azepines)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Pyridinium Compounds)
RN  - 0 (Triazoles)
RN  - 100488-87-7 (CV 6209)
RN  - 105219-56-5 (WEB 2086)
RN  - 11062-77-4 (Superoxides)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 886U3H6UFF (Chloroquine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/blood
MH  - Azepines/pharmacology
MH  - Blood Platelets/drug effects/*physiology
MH  - Calcimycin/pharmacology
MH  - Chloroquine/pharmacology
MH  - Female
MH  - Kinetics
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/drug effects/*physiology
MH  - Platelet Activating Factor/antagonists & inhibitors/pharmacology/*physiology
MH  - Pyridinium Compounds/pharmacology
MH  - Rabbits
MH  - *Signal Transduction/drug effects
MH  - Superoxides/blood
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Triazoles/pharmacology
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
AID - 10.1007/BF02536499 [doi]
PST - ppublish
SO  - Lipids. 1991 Dec;26(12):1044-9. doi: 10.1007/BF02536499.