PMID- 16682787
OWN - NLM
STAT- MEDLINE
DCOM- 20060829
LR  - 20190911
IS  - 1347-8613 (Print)
IS  - 1347-8613 (Linking)
VI  - 101
IP  - 1
DP  - 2006 May
TI  - Development and evaluation of a direct sandwich enzyme-linked immunosorbent assay 
      for the quantification of guinea-pig immunoglobulin e.
PG  - 58-65
AB  - The purpose of this study was to develop and evaluate a direct sandwich 
      enzyme-linked immunosorbent assay (ELISA) for the immunoglobulin E (IgE) in serum 
      and plasma from guinea pig using mouse monoclonal antibodies specific for 
      guinea-pig IgE. Mouse monoclonal antibodies were raised against purified IgE 
      protein. The ELISA was performed using a combination of two anti-IgE monoclonal 
      antibodies. One antibody was labeled with horseradish peroxidase (HRP), and the 
      other was coated on polystyrene wells. Purified guinea-pig IgE was used as the 
      standard material. The validity of the ELISA was confirmed by precision, 
      dilution, recovery, and interference tests. The range of detection was 3.1-800 ng 
      of IgE mass per mL of serum and plasma. The intra- and inter-assay coefficients 
      of variation were 4.6% and 5.7%, respectively, or less. The recovery test showed 
      variation only between 92.1% and 111.8%, and the anticoagulants showed 
      noninterference with the IgE assay. The mean serum IgE mass concentration in 
      OVA-sensitized guinea pigs was 29438 ng/mL, and it was 48.6 ng/mL in normal 
      guinea pigs. The present ELISA is useful and practical for specific measurement 
      of the guinea-pig IgE, and it is surmised that it would be suitable for use in 
      allergological and pharmacological research.
FAU - Nishimura, Makoto
AU  - Nishimura M
AD  - Dainippon Sumitomo Pharma Co., Ltd., Suita, Osaka, Japan. 
      makoto-nishimura@ds-pharma.co.jp
FAU - Funaoka, Hiroyuki
AU  - Funaoka H
FAU - Hosoe, Hiroaki
AU  - Hosoe H
FAU - Ohkaru, Yasuhiko
AU  - Ohkaru Y
FAU - Yakuo, Ikuhisa
AU  - Yakuo I
FAU - Hayakawa, Shinobu
AU  - Hayakawa S
FAU - Ito, Koji
AU  - Ito K
LA  - eng
PT  - Journal Article
DEP - 20060509
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Antibodies, Monoclonal)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Cross Reactions
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Guinea Pigs/*immunology
MH  - Hybridomas
MH  - Immunoglobulin E/*blood
MH  - Sensitivity and Specificity
EDAT- 2006/05/10 09:00
MHDA- 2006/08/30 09:00
CRDT- 2006/05/10 09:00
PHST- 2006/05/10 09:00 [pubmed]
PHST- 2006/08/30 09:00 [medline]
PHST- 2006/05/10 09:00 [entrez]
AID - JST.JSTAGE/jphs/FPJ05033X [pii]
AID - 10.1254/jphs.fpj05033x [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2006 May;101(1):58-65. doi: 10.1254/jphs.fpj05033x. Epub 2006 
      May 9.