PMID- 16682819
OWN - NLM
STAT- MEDLINE
DCOM- 20070206
LR  - 20240315
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 21
IP  - 2
DP  - 2006 Apr 30
TI  - Nerve growth factor activates brain-derived neurotrophic factor promoter IV via 
      extracellular signal-regulated protein kinase 1/2 in PC12 cells.
PG  - 237-43
AB  - Brain-derived neurotrophic factor (BDNF) is a neuromodulator of nociceptive 
      responses in the dorsal root ganglia (DRG) and spinal cord. BDNF synthesis 
      increases in response to nerve growth factor (NGF) in trkA-expressing small and 
      medium-sized DRG neurons after inflammation. Previously we demonstrated 
      differential activation of multiple BDNF promoters in the DRG following 
      peripheral nerve injury and inflammation. Using reporter constructs containing 
      individual promoter regions, we investigated the effect of NGF on the multiple 
      BDNF promoters, and the signaling pathway by which NGF activates these promoters 
      in PC12 cells. Although all the promoters were activated 2.4-7.1-fold by NGF 
      treatment, promoter IV gave the greatest induction. The p38 mitogen-activated 
      protein kinase (MAPK) inhibitor, SB203580, phosphatidylinositol 3-kinase (PI-3K) 
      inhibitor, LY294003, protein kinase A (PKA) inhibitor, H89, and protein kinase C 
      (PKC) inhibitor, chelerythrine, had no effect on activation of promoter IV by 
      NGF. However, activation was completely abolished by the MAPK kinase (MEK) 
      inhibitors, U0126 and PD98059. In addition, these inhibitors blocked NGF-induced 
      phosphorylation of extracellular signal-regulated protein kinase (ERK) 1/2. Taken 
      together, these results suggest that the ERK1/2 pathway activates BDNF promoter 
      IV in response to NGF independently of NGF-activated signaling pathways involving 
      PKA and PKC.
FAU - Park, So Yun
AU  - Park SY
AD  - Department of Anatomy, School of Medicine, Kyungpook National University, Daegu 
      700-422, Korea.
FAU - Lee, Ji Yun
AU  - Lee JY
FAU - Choi, Jun Young
AU  - Choi JY
FAU - Park, Mae Ja
AU  - Park MJ
FAU - Kim, Dong Sun
AU  - Kim DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Butadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Nitriles)
RN  - 0 (U 0126)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - *Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Butadienes/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Enzyme Inhibitors/metabolism
MH  - Flavonoids/metabolism
MH  - Genes, Reporter
MH  - Mitogen-Activated Protein Kinase 1/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors/metabolism
MH  - Nerve Growth Factor/*metabolism
MH  - Nitriles/metabolism
MH  - PC12 Cells
MH  - *Promoter Regions, Genetic
MH  - Protein Kinase C/metabolism
MH  - Rats
MH  - Signal Transduction/physiology
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
EDAT- 2006/05/10 09:00
MHDA- 2007/02/07 09:00
CRDT- 2006/05/10 09:00
PHST- 2006/05/10 09:00 [pubmed]
PHST- 2007/02/07 09:00 [medline]
PHST- 2006/05/10 09:00 [entrez]
AID - S1016-8478(23)12886-X [pii]
PST - ppublish
SO  - Mol Cells. 2006 Apr 30;21(2):237-43.