PMID- 16682974
OWN - NLM
STAT- MEDLINE
DCOM- 20060920
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 86
IP  - 8
DP  - 2006 Aug
TI  - Function-blocking autoantibodies to the melanin-concentrating hormone receptor in 
      vitiligo patients.
PG  - 781-9
AB  - Vitiligo is a common depigmenting skin disorder resulting from the loss of 
      melanocytes in the cutaneous epidermis. Although the cause of the disease remains 
      obscure, autoimmune mechanisms are thought to be involved. Recently, 
      melanin-concentrating hormone receptor (MCHR)-binding autoantibodies have been 
      identified in vitiligo patients. In the present study, we aimed to determine if 
      MCHR autoantibodies could also affect receptor function either by direct 
      activation or by blocking its response to melanin-concentrating hormone. The 
      results indicated that 10/18 (56%) vitiligo patient IgG samples inhibited the 
      function of MCHR expressed in a Chinese hamster ovary cell line. In contrast, 
      neither control (n=20) nor SLE patient (n=10) IgG samples blocked receptor 
      function. Compared with healthy controls, MCHR function-blocking autoantibodies 
      were found at a significantly increased frequency in the vitiligo patient group 
      (P=0.0004). No MCHR-activating autoantibodies were detected in any of the 
      vitiligo patient, SLE patient or control IgG samples that were analysed. In 
      addition, vitiligo patient IgGs were tested for MCHR autoantibodies that could 
      mediate antibody-dependent cell-mediated cytotoxicity via the receptor. However, 
      this could only be demonstrated in two vitiligo patient sera. Overall, this work 
      has provided additional evidence that MCHR is a B-cell autoantigen in vitiligo 
      and has demonstrated the existence of MCHR function-blocking autoantibodies 
      further to the receptor-binding autoantibodies previously reported.
FAU - Gottumukkala, Raju V S R K
AU  - Gottumukkala RV
AD  - Division of Clinical Sciences (North), University of Sheffield, Sheffield, UK.
FAU - Gavalas, Nikos G
AU  - Gavalas NG
FAU - Akhtar, Samia
AU  - Akhtar S
FAU - Metcalfe, Russell A
AU  - Metcalfe RA
FAU - Gawkrodger, David J
AU  - Gawkrodger DJ
FAU - Haycock, John W
AU  - Haycock JW
FAU - Watson, Philip F
AU  - Watson PF
FAU - Weetman, Anthony P
AU  - Weetman AP
FAU - Kemp, Elizabeth Helen
AU  - Kemp EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060508
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Pituitary Hormone)
RN  - 0 (melanin-concentrating hormone receptor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Antibody-Dependent Cell Cytotoxicity
MH  - Autoantibodies/*immunology
MH  - CHO Cells
MH  - Case-Control Studies
MH  - Cricetinae
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Middle Aged
MH  - Receptors, Pituitary Hormone/*immunology
MH  - Vitiligo/*immunology
EDAT- 2006/05/10 09:00
MHDA- 2006/09/21 09:00
CRDT- 2006/05/10 09:00
PHST- 2006/05/10 09:00 [pubmed]
PHST- 2006/09/21 09:00 [medline]
PHST- 2006/05/10 09:00 [entrez]
AID - S0023-6837(22)01798-6 [pii]
AID - 10.1038/labinvest.3700438 [doi]
PST - ppublish
SO  - Lab Invest. 2006 Aug;86(8):781-9. doi: 10.1038/labinvest.3700438. Epub 2006 May 
      8.