PMID- 16684508
OWN - NLM
STAT- MEDLINE
DCOM- 20060711
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 345
IP  - 1
DP  - 2006 Jun 23
TI  - Epidermal-type fatty acid binding protein as a negative regulator of IL-12 
      production in dendritic cells.
PG  - 459-66
AB  - Fatty acids and their metabolites have recently been shown to modulate various 
      functions of dendritic cells (DCs) including their differentiation and cytokine 
      production, although the mechanisms underlying their cellular functions are not 
      fully understood. In view of our previous finding that epidermal-type fatty acid 
      binding protein (E-FABP) was exclusively expressed in splenic DCs among FABP 
      family, we examined the phenotype of E-FABP-null mutant mice in order to 
      elucidate the functional significance of E-FABP expression in DCs. Although 
      E-FABP-null mutant mice showed no apparent abnormalities in the population 
      density and subset distribution of DCs as well as the microscopic morphology in 
      the spleen, DCs isolated from E-FABP-null spleen showed enhanced production of 
      IL-12p70, a key cytokine for innate immune responses, in response to appropriate 
      stimuli as compared with wild-type. In real-time PCR, the expression level of 
      IL-12p35 mRNA after LPS stimuli was much higher in mutant DCs when compared with 
      wild-type, while no apparent change of IL-12p40 mRNA level was detected. 
      Phosphorylated forms of p38 mitogen-activated protein kinase (p38MAPK) and 
      IkappaB-alpha, molecules critical for IL-12 production, were detected at higher 
      levels in E-FABP-null-mutant DCs after LPS stimuli when compared with wild-type 
      counterparts. Collectively, it is suggested that E-FABP may be a novel negative 
      regulator of IL-12 production in DCs, and this regulation may be exerted via its 
      involvement in the p38MAPK-mediated transcription of IL-12p35.
FAU - Kitanaka, Noriko
AU  - Kitanaka N
AD  - Department of Histology, Graduate School of Medicine, Tohoku University, Japan.
FAU - Owada, Yuji
AU  - Owada Y
FAU - Okuyama, Ryuhei
AU  - Okuyama R
FAU - Sakagami, Hiroyuki
AU  - Sakagami H
FAU - Nourani, Mohammad Reza
AU  - Nourani MR
FAU - Aiba, Setsuya
AU  - Aiba S
FAU - Furukawa, Hiroshi
AU  - Furukawa H
FAU - Watanabe, Makoto
AU  - Watanabe M
FAU - Ono, Masao
AU  - Ono M
FAU - Ohteki, Toshiaki
AU  - Ohteki T
FAU - Kondo, Hisatake
AU  - Kondo H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060502
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (FABP5 protein, human)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - Down-Regulation/physiology
MH  - Fatty Acid-Binding Proteins/genetics/*metabolism
MH  - Interleukin-12/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
EDAT- 2006/05/11 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/05/11 09:00
PHST- 2006/04/08 00:00 [received]
PHST- 2006/04/17 00:00 [accepted]
PHST- 2006/05/11 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/05/11 09:00 [entrez]
AID - S0006-291X(06)00891-6 [pii]
AID - 10.1016/j.bbrc.2006.04.114 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Jun 23;345(1):459-66. doi: 
      10.1016/j.bbrc.2006.04.114. Epub 2006 May 2.