PMID- 16685390
OWN - NLM
STAT- MEDLINE
DCOM- 20070308
LR  - 20060510
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 15
IP  - 6
DP  - 2006 Jun
TI  - G-CSF and/or M-CSF accelerate differentiation of bone marrow cells into 
      endothelial progenitor cells in vitro.
PG  - 1523-7
AB  - It has been reported that granulocyte-colony stimulating factor (G-CSF) and 
      granulocyte-macrophage-colony stimulating factor (GM-CSF) can mobilize 
      endothelial progenitor cells (EPCs) in bone marrow cells (BMCs) into peripheral 
      blood (PB) in vivo. Previously, we also reported that macrophage-colony 
      stimulating factor (M-CSF) can mobilize EPCs into PB, which results in the rapid 
      recovery of blood flow in induced-ischemia limbs by augmenting the number of 
      intramuscular capillaries in vivo. In the present study, we demonstrate that 
      M-CSF and/or G-CSF can increase EPCs from lineage (CD3, B220, Gr-1, Mac-1, CD11c, 
      Ter119, NK1.1 or CD31)-negative BMCs in vitro. Lineage-negative BMCs were 
      cultured with or without M-CSF and/or G-CSF. Three days after culture with M-CSF 
      and/or G-CSF, the number of Flk-1+/CD45-, Sca-1+/CD45-, CD31+/CD45- or 
      CD146+/CD45- cells increased in comparison with no cytokines. When the cultured 
      BMCs with or without G-CSF and/or M-CSF were intravenously injected into 
      ischemia-induced hindlimbs of mice, the number of intramuscular capillaries in 
      the ischemia-induced legs increased; BMCs cultured with G-CSF and/or M-CSF were 
      more effective than those of cytokine non-treated BMCs. These results suggest 
      that M-CSF and/or G-CSF can induce the differentiation of BMCs into EPCs, even in 
      vitro.
FAU - Zhang, Yuming
AU  - Zhang Y
AD  - First Department of Pathology, Kansai Medical University, Moriguchi, Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Iwasaki, Masayoshi
AU  - Iwasaki M
FAU - Minamino, Keizo
AU  - Minamino K
FAU - Suzuki, Yasuhiro
AU  - Suzuki Y
FAU - Nakano, Keiji
AU  - Nakano K
FAU - Koike, Yasushi
AU  - Koike Y
FAU - Mukaide, Hiromi
AU  - Mukaide H
FAU - Shigematsu, Akio
AU  - Shigematsu A
FAU - Kiriyama, Naoko
AU  - Kiriyama N
FAU - Li, Chunfu
AU  - Li C
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Lineage
MH  - Endothelial Cells/cytology/*drug effects
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - Hindlimb/blood supply
MH  - Ischemia/drug therapy
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Stem Cells/cytology/*drug effects
EDAT- 2006/05/11 09:00
MHDA- 2007/03/09 09:00
CRDT- 2006/05/11 09:00
PHST- 2006/05/11 09:00 [pubmed]
PHST- 2007/03/09 09:00 [medline]
PHST- 2006/05/11 09:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2006 Jun;15(6):1523-7.