PMID- 16687586
OWN - NLM
STAT- MEDLINE
DCOM- 20060710
LR  - 20200403
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 55
IP  - Pt 6
DP  - 2006 Jun
TI  - Human herpesvirus-6 dysregulates monocyte-mediated anticryptococcal defences.
PG  - 695-702
LID - 10.1099/jmm.0.46496-0 [doi]
AB  - In order to investigate the interplay occurring between pathogens in the course 
      of double infections, an in vitro model was set up in which the monocytic cell 
      line THP-1 was exposed to Cryptococcus neoformans (Cn) and human herpesvirus 6 
      (HHV-6). Cn and HHV-6, both highly neurotropic, can cause serious diseases of the 
      central nervous system and have monocytes, among other cell types, as target 
      cells, causing alteration of their secretion pattern. Here, it was shown that 
      unlike THP-1 cells exposed to cell-free virus inocula, THP-1 exposed to 
      HHV-6-producing lymphocytes exhibited augmented phagocytosis against Cn. The 
      phenomenon occurred after 24 h of monocyte/lymphocyte co-culture and was 
      independent of direct cell-to-cell contact. Moreover, in the presence of HHV-6, 
      THP-1 cells expressed enhanced secretory responses but reduced capability to 
      counteract fungal infection: the enhanced ingestion by monocytes was followed by 
      facilitated fungal survival and replication. These data provide initial in vitro 
      evidence that HHV-6 may dysregulate monocyte-mediated anticryptococcal defences 
      with an overall pro-cryptococcus result.
FAU - Cermelli, Claudio
AU  - Cermelli C
AD  - Department of Hygiene, Microbiology and Biostatistics, University of Modena and 
      Reggio Emilia, Via Campi 287, I-41100 Modena, Italy.
FAU - Cenacchi, Valeria
AU  - Cenacchi V
AD  - Department of Hygiene, Microbiology and Biostatistics, University of Modena and 
      Reggio Emilia, Via Campi 287, I-41100 Modena, Italy.
FAU - Beretti, Francesca
AU  - Beretti F
AD  - Department of Hygiene, Microbiology and Biostatistics, University of Modena and 
      Reggio Emilia, Via Campi 287, I-41100 Modena, Italy.
FAU - Pezzini, Francesco
AU  - Pezzini F
AD  - Department of Hygiene, Microbiology and Biostatistics, University of Modena and 
      Reggio Emilia, Via Campi 287, I-41100 Modena, Italy.
FAU - Luca, Dario Di
AU  - Luca DD
AD  - Department of Experimental and Diagnostic Medicine, University of Ferrara, 
      Ferrara, Italy.
FAU - Blasi, Elisabetta
AU  - Blasi E
AD  - Department of Hygiene, Microbiology and Biostatistics, University of Modena and 
      Reggio Emilia, Via Campi 287, I-41100 Modena, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Interferon-alpha)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Cell Line
MH  - Coculture Techniques
MH  - Cryptococcosis/complications/immunology
MH  - Cryptococcus neoformans/immunology/*pathogenicity
MH  - Herpesvirus 6, Human/*pathogenicity
MH  - Humans
MH  - Interferon-alpha/biosynthesis
MH  - Interleukin-12/biosynthesis
MH  - Monocytes/immunology/*microbiology/*virology
MH  - Phagocytosis
MH  - Phenotype
MH  - Roseolovirus Infections/complications/immunology
EDAT- 2006/05/12 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/05/12 09:00
PHST- 2006/05/12 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/05/12 09:00 [entrez]
AID - 10.1099/jmm.0.46496-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2006 Jun;55(Pt 6):695-702. doi: 10.1099/jmm.0.46496-0.