PMID- 16691201
OWN - NLM
STAT- MEDLINE
DCOM- 20060905
LR  - 20161124
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 126
IP  - 8
DP  - 2006 Aug
TI  - Diminished induction of skin fibrosis in mice with MCP-1 deficiency.
PG  - 1900-8
AB  - Scar and fibrosis are often the end result of mechanical injury and inflammatory 
      diseases. One chemokine that is repeatedly linked to fibrotic responses is 
      monocyte chemoattractant protein-1 (MCP-1). We utilized a murine fibrosis model 
      that produces dermal lesions similar to scleroderma to evaluate collagen 
      fibrillogenesis in the absence of MCP-1. Dermal fibrosis was induced by 
      subcutaneous injection of bleomycin into the dorsal skin of MCP-1-/- and 
      wild-type C57BL/6 mice. After 4 weeks of daily injections, bleomycin treatment 
      led to thickened collagen bundles with robust inflammation in the lesional dermis 
      of wild-type mice. In contrast, the lesional skin of MCP-1-/- mice exhibited a 
      dermal architecture similar to phosphate-buffered saline (PBS)-injected control 
      and normal skin, with few inflammatory cells. Ultrastructural analysis of the 
      lesional dermis from bleomycin-injected wild-type mice revealed markedly abnormal 
      arrangement of collagen fibrils, with normal large diameter collagen fibrils 
      replaced by small collagen fibrils of 41.5 nm. In comparison, the dermis of 
      bleomycin-injected MCP-1-/- mice displayed a uniform pattern of fibril diameters 
      that was similar to normal skin (average diameter 76.7 nm). The findings 
      implicate MCP-1 as a key determinant in the development of skin fibrosis induced 
      by bleomycin, and suggest that MCP-1 may influence collagen fiber formation in 
      vivo.
FAU - Ferreira, Ahalia M
AU  - Ferreira AM
AD  - Department of Surgery, The Burn and Shock Trauma Institute, Loyola University 
      Medical Center, Maywood, Illinois, USA.
FAU - Takagawa, Shinsuke
AU  - Takagawa S
FAU - Fresco, Raoul
AU  - Fresco R
FAU - Zhu, Xiaofeng
AU  - Zhu X
FAU - Varga, John
AU  - Varga J
FAU - DiPietro, Luisa A
AU  - DiPietro LA
LA  - eng
GR  - R01GM55238/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060511
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Collagen Type I)
RN  - 0 (Dcn protein, mouse)
RN  - 0 (Decorin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fmod protein, mouse)
RN  - 0 (HSP47 Heat-Shock Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (Serpinh1 protein, mouse)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 11056-06-7 (Bleomycin)
RN  - 126468-95-9 (Fibromodulin)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Mmp13 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antimetabolites, Antineoplastic
MH  - Bleomycin
MH  - Chemokine CCL2/deficiency/*genetics/metabolism
MH  - Collagen Type I/genetics/metabolism/ultrastructure
MH  - Collagenases/metabolism
MH  - Decorin
MH  - Dermis/*pathology/physiopathology
MH  - Disease Models, Animal
MH  - Extracellular Matrix Proteins/genetics
MH  - Fibroblasts/pathology/ultrastructure
MH  - Fibromodulin
MH  - Fibrosis
MH  - Gene Expression
MH  - HSP47 Heat-Shock Proteins/genetics
MH  - Matrix Metalloproteinase 13
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Proteoglycans/genetics
MH  - Skin Diseases/chemically induced/*pathology/*physiopathology
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - Transforming Growth Factor beta/metabolism
MH  - Transforming Growth Factor beta1
EDAT- 2006/05/13 09:00
MHDA- 2006/09/06 09:00
CRDT- 2006/05/13 09:00
PHST- 2006/05/13 09:00 [pubmed]
PHST- 2006/09/06 09:00 [medline]
PHST- 2006/05/13 09:00 [entrez]
AID - S0022-202X(15)33003-7 [pii]
AID - 10.1038/sj.jid.5700302 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2006 Aug;126(8):1900-8. doi: 10.1038/sj.jid.5700302. Epub 2006 
      May 11.