PMID- 16697051
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20161124
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 175
IP  - 1-2
DP  - 2006 Jun
TI  - Myasthenia gravis induced in mice by immunization with the recombinant 
      extracellular domain of rat muscle-specific kinase (MuSK).
PG  - 107-17
AB  - Myasthenia gravis (MG) is mostly caused by anti-acetylcholine receptor (AChR) 
      auto-antibodies (Abs). Such Abs are undetectable in 10-15% of MG patients, but 
      many have anti-muscle-specific kinase (MuSK) Abs. We injected recombinant 
      rat-MuSK extracellular domain in H-2(a), H-2(b), H-2(bm12) and H-2(d) mice. 
      Certain strains exhibited exercise-induced fatigue, tremors, weight loss, and 
      some died after 2-3 injections. Compound muscle action potentials showed 
      decrement with low-frequency repetitive nerve stimulation. Miniature endplate 
      potentials decreased, suggesting lower numbers of endplates functional AChRs. 
      Myasthenic sera inhibited agrin-induced AChR aggregation in C2C12 myotubes. 
      CONCLUSION: Anti-MuSK Abs induce MG, which might also result from blocking the 
      agrin-signaling pathway.
FAU - Jha, Smita
AU  - Jha S
AD  - Department of Biology and Biochemistry, University of Houston, TX 77204, USA.
FAU - Xu, Kaiping
AU  - Xu K
FAU - Maruta, Takahiro
AU  - Maruta T
FAU - Oshima, Minako
AU  - Oshima M
FAU - Mosier, Dennis R
AU  - Mosier DR
FAU - Atassi, M Zouhair
AU  - Atassi MZ
FAU - Hoch, Werner
AU  - Hoch W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060511
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Autoantibodies)
RN  - 0 (Receptors, Cholinergic)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (MuSK protein, rat)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Action Potentials/immunology
MH  - Animals
MH  - Autoantibodies/biosynthesis/blood
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Extracellular Fluid/*enzymology/immunology
MH  - Female
MH  - Immunization
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Muscle, Skeletal/immunology/metabolism
MH  - Myasthenia Gravis/*enzymology/*immunology
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Receptor Protein-Tyrosine Kinases/*administration & dosage/*immunology
MH  - Receptors, Cholinergic/*administration & dosage/*immunology
MH  - Recombinant Proteins/administration & dosage/immunology
EDAT- 2006/05/16 09:00
MHDA- 2006/07/27 09:00
CRDT- 2006/05/16 09:00
PHST- 2005/12/01 00:00 [received]
PHST- 2006/02/08 00:00 [revised]
PHST- 2006/03/10 00:00 [accepted]
PHST- 2006/05/16 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/05/16 09:00 [entrez]
AID - S0165-5728(06)00086-5 [pii]
AID - 10.1016/j.jneuroim.2006.03.016 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2006 Jun;175(1-2):107-17. doi: 10.1016/j.jneuroim.2006.03.016. 
      Epub 2006 May 11.