PMID- 16698426
OWN - NLM
STAT- MEDLINE
DCOM- 20060829
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 67
IP  - 1-2
DP  - 2006 Jan-Feb
TI  - Polymorphism in the 5' upstream regulatory and 3' untranslated regions of the 
      HLA-G gene in relation to soluble HLA-G and IL-10 expression.
PG  - 53-62
AB  - The nonclassical human leukocyte antigen (HLA) class Ib gene HLA-G may be 
      important for the induction and maintenance of immune tolerance between the 
      mother and the semi-allogeneic fetus during pregnancy. Expression of HLA-G can 
      influence cytokine and cytotoxic T-lymphocyte responses. Different HLA-G mRNA 
      isoform expression patterns have been associated with HLA-G polymorphism, 
      especially with a 14-bp insertion deletion polymorphism in the 3' untranslated 
      region (3'UTR) of the HLA-G gene. A significantly high level of interleukin-10 
      (IL-10) secretion is observed in homozygous +14/+14-bp HLA-G peripheral blood 
      mononuclear cells after lipopolysaccharide (LPS) stimulation. This study finds 
      that polymorphism in the 5' upstream regulatory region (5'URR) of the HLA-G gene 
      may also be implicated in differences in IL-10 secretion. However, this may also 
      be due to linkage disequilibrium with the 14-bp polymorphism. A single-nucleotide 
      polymorphism located -477 bp from the start site of exon 1 had a significant 
      association with IL-10 concentrations but not after correction (p=0.011; 
      pc=0.154). This polymorphism is located next to a heat shock element. Eighteen 
      5'-URR/3'-UTR HLA-G haplotypes were defined; one common homozygous genotype based 
      on these haplotypes was significantly associated with a high IL-10 level after 
      LPS stimulation compared to certain other genotypes. This study indicates that 
      polymorphism in the 5'-URR of the HLA-G gene may have functional significance, 
      although a new line of investigations is needed to elucidate these findings.
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TV
AD  - Department of Clinical Biochemistry, Roskilde University Hospital, Roskilde, 
      Denmark.
FAU - Rizzo, Roberta
AU  - Rizzo R
FAU - Melchiorri, Loredana
AU  - Melchiorri L
FAU - Stignani, Marina
AU  - Stignani M
FAU - Baricordi, Olavio R
AU  - Baricordi OR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060331
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Lipopolysaccharides)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - 3' Untranslated Regions/*genetics
MH  - Base Sequence
MH  - Gene Expression
MH  - HLA Antigens/*genetics
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Interleukin-10/*metabolism
MH  - Leukocytes, Mononuclear/drug effects/immunology
MH  - Linkage Disequilibrium
MH  - Lipopolysaccharides/pharmacology
MH  - Molecular Sequence Data
MH  - *Polymorphism, Genetic
MH  - Regulatory Elements, Transcriptional/*genetics
EDAT- 2006/05/16 09:00
MHDA- 2006/08/30 09:00
CRDT- 2006/05/16 09:00
PHST- 2005/09/26 00:00 [received]
PHST- 2006/05/16 09:00 [pubmed]
PHST- 2006/08/30 09:00 [medline]
PHST- 2006/05/16 09:00 [entrez]
AID - S0198-8859(05)00468-4 [pii]
AID - 10.1016/j.humimm.2005.12.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2006 Jan-Feb;67(1-2):53-62. doi: 10.1016/j.humimm.2005.12.003. Epub 
      2006 Mar 31.