PMID- 16699557
OWN - NLM
STAT- MEDLINE
DCOM- 20060725
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 4
DP  - 2006 Apr
TI  - Karyological analysis of an interspecific hybrid between the dioecious Silene 
      latifolia and the hermaphroditic Silene viscosa.
PG  - 373-9
AB  - The genus Silene is a good model for studying evolution of the sex chromosomes, 
      since it includes species that are hermaphroditic and dioecious, while maintain a 
      basic chromosome number of 2n = 24. For some combinations of Silene species it is 
      possible to construct interspecific hybrids. Here, we present a detailed 
      karyological analysis of a hybrid between the dioecious Silene latifolia as the 
      maternal plant and a related species, hermaphroditic Silene viscosa, used as a 
      pollen partner. Using genomic probes (the genomic in situ hybridization (GISH) 
      technique), we were able to clearly discriminate parental genomes and to show 
      that they are largely separated in distinct nuclear domains. Molecular GISH and 
      fluorescence in situ hybridization (FISH) markers document that the hybrid genome 
      of somatic cells was strictly additive and stable, and that it had 12 chromosomes 
      originating from each parent, including the only X chromosome of S. latifolia. 
      Meiotic analysis revealed that, although related, respective parental chromosomes 
      did not pair or paired only partially, which resulted in frequent chromosome 
      abnormalities such as bridges and irregular non-disjunctions. GISH and FISH 
      markers clearly document that the larger genome of S. latifolia and its largest 
      chromosome component, the X chromosome, were mostly employed in chromosome 
      lagging and misdivision.
FAU - Markova, Michaela
AU  - Markova M
AD  - Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of 
      Sciences of the Czech Republic, Kralovopolska 135, CZ-61265 Brno, Czech Republic.
FAU - Lengerova, Martina
AU  - Lengerova M
FAU - Zluvova, Jitka
AU  - Zluvova J
FAU - Janousek, Bohuslav
AU  - Janousek B
FAU - Vyskot, Boris
AU  - Vyskot B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Chimera/*genetics
MH  - Chromosome Segregation/genetics
MH  - Chromosomes, Plant/ultrastructure
MH  - *Crosses, Genetic
MH  - Disorders of Sex Development/*genetics
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/*methods
MH  - Meiosis/genetics
MH  - Phylogeny
MH  - Silene/*genetics
MH  - X Chromosome/ultrastructure
EDAT- 2006/05/16 09:00
MHDA- 2006/07/26 09:00
CRDT- 2006/05/16 09:00
PHST- 2006/05/16 09:00 [pubmed]
PHST- 2006/07/26 09:00 [medline]
PHST- 2006/05/16 09:00 [entrez]
AID - g05-112 [pii]
AID - 10.1139/g05-112 [doi]
PST - ppublish
SO  - Genome. 2006 Apr;49(4):373-9. doi: 10.1139/g05-112.