PMID- 16701011
OWN - NLM
STAT- MEDLINE
DCOM- 20060608
LR  - 20081121
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 119
IP  - 9
DP  - 2006 May 5
TI  - Impact of genetic variation of tumor necrosis factor-alpha on gestational 
      hypertension.
PG  - 719-24
AB  - BACKGROUND: The mechanisms responsible for the pathogeneses of gestational 
      hypertension and preeclampsia are unclear. Tumor necrosis factor-alpha 
      (TNF-alpha) is a pro-inflammatory Th(1)-type cytokine. TNFA gene is located in 
      the human leukocyte antigen (HLA) class III region of the major 
      histocompatibility complex (MHC) on chromosome 6. The high TNF-alpha mRNA 
      expression may be associated with the TNF2 (A) allele, which is the polymorphism 
      of TNF-alpha at position -308 in promoter region. This study assessed whether the 
      TNF2 (A) allele at position -308 plays a role in the alteration of blood pressure 
      (BP) and urinary protein excretion during pregnancy. METHODS: The original 
      prospective cohort study comprised 1623 pregnant women from January 2000 to 
      October 2001. The G/A polymorphism was done by restriction fragment length 
      polymorphism (RFLP) analysis with Nco I enzyme. RESULTS: The distributions of the 
      G/A polymorphism of TNF-alpha in the promoter region at position -308 were 
      wild-type 72.4% and variant 27.6%, respectively. The frequency of TNF2 (A) allele 
      was approximately 0.15 for Caucasian pregnant women in the study. It was not 
      significantly different in the distributions of genotypes and G/A allele 
      frequencies among the three groups of pregnant women with gestational 
      hypertension, preexisting hypertension and normal blood pressure (P > 0.05). The 
      maternal blood pressure in the third trimester was significantly higher in the 
      group of women possessing the TNF2 (A) allele compared to homozygous for the TNF1 
      (G) allele (systolic BP, P < 0.01 and diastolic BP, P < 0.05). The elevated blood 
      pressure in the TNF2 (A) group was accompanied by higher urinary protein 
      excretion in the third trimester (P < 0.05). The blood pressure and urinary 
      protein excretion did not change apparently between the two groups in the first 
      and second trimesters (P > 0.05). CONCLUSIONS: Maternal TNF2 (A) allele of 
      TNF-alpha promoter region at position -308 could play a role in the alteration of 
      blood pressures and/or enhancement of urinary protein excretion during pregnancy, 
      and might play an important role in the development of both gestational 
      hypertension and preeclampsia.
FAU - Chen, You-peng
AU  - Chen YP
AD  - Division of Infectious Disease, Department of Interal Medicine, First Affiliated 
      Hospital of Jinan University, Jinan University, Guangzhou 510632, China.
FAU - Pfab, Thiemo
AU  - Pfab T
FAU - Slowinski, Torsten
AU  - Slowinski T
FAU - Richter, Claus-Michael
AU  - Richter CM
FAU - Godes, Michael
AU  - Godes M
FAU - Hocher, Berthold
AU  - Hocher B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cohort Studies
MH  - Female
MH  - Genetic Variation
MH  - Humans
MH  - Hypertension, Pregnancy-Induced/*genetics
MH  - Middle Aged
MH  - *Polymorphism, Genetic
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - Prospective Studies
MH  - Proteinuria/genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2006/05/17 09:00
MHDA- 2006/06/09 09:00
CRDT- 2006/05/17 09:00
PHST- 2006/05/17 09:00 [pubmed]
PHST- 2006/06/09 09:00 [medline]
PHST- 2006/05/17 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2006 May 5;119(9):719-24.