PMID- 16704298
OWN - NLM
STAT- MEDLINE
DCOM- 20060626
LR  - 20231213
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 26
IP  - 4
DP  - 2006 Apr
TI  - OAS and PKR are not required for the antiviral effect of Ad:IFN-gamma against 
      acute HSV-1 in primary trigeminal ganglia cultures.
PG  - 220-5
AB  - Three interferon-gamma (IFN-gamma)-induced antiviral pathways have been reported. 
      Involved antiviral proteins include: Mx, RNase L/2',5'-OAS, and protein kinase R 
      (PKR). Involvement of OAS and PKR in IFN-gamma-induced anti-herpes simplex 
      virus-1 (HSV-1) pathways has not been reported previously, but IFN-gamma induces 
      OAS and PKR when other viruses invade the nervous system. The aim of the current 
      study was to determine if the absence of intact OAS and PKR antiviral pathways 
      affects the antiviral activity of IFN-gamma during acute HSV-1 infection within 
      the trigeminal ganglia (TG). To investigate this, primary TG cultures were 
      established using TGs removed from C57BL/6 (wild-type), RNase L knockout, and 
      RNase L/PKR double knockout mice. Each dissociated TG was transduced with an 
      adenoviral vector containing an IFN-gamma transgene or vector alone. Viral titers 
      after HSV-1 infection of primary TG cell cultures were determined. Significant 
      differences in viral titer for Ad:Null-transduced vs. Ad:IFN-gamma-tranduced TG 
      were found in each genotype. However, the effectiveness of Ad:IFN-gamma was not 
      reduced in the absence of both OAS and PKR pathways or OAS alone. Recombinant 
      IFN-gamma also exhibited anti-HSV-1 activity. The effectiveness of the IFN-gamma 
      transgene was lost in primary TG cells from IFN-gamma receptor knockout mice. The 
      data suggest that novel anti-HSV-1 mechanisms are induced by IFN-gamma.
FAU - Austin, Bobbie Ann
AU  - Austin BA
AD  - Department of Ophthalmology, University of Oklahoma Health Sciences Center, 
      Oklahoma City, OK 73104, USA.
FAU - Halford, William
AU  - Halford W
FAU - Silverman, Robert H
AU  - Silverman RH
FAU - Williams, Bryan R G
AU  - Williams BR
FAU - Carr, Daniel J J
AU  - Carr DJ
LA  - eng
GR  - R01 AI053108/AI/NIAID NIH HHS/United States
GR  - R01 AI034039/AI/NIAID NIH HHS/United States
GR  - P30 EY012190/EY/NEI NIH HHS/United States
GR  - EY12190/EY/NEI NIH HHS/United States
GR  - AI34039/AI/NIAID NIH HHS/United States
GR  - CA44059/CA/NCI NIH HHS/United States
GR  - R01 AI051414/AI/NIAID NIH HHS/United States
GR  - R01 CA044059/CA/NCI NIH HHS/United States
GR  - R01 AI051414-03/AI/NIAID NIH HHS/United States
GR  - AI053108/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Receptors, Interferon)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.11.1 (eIF-2 Kinase)
RN  - EC 2.7.7.84 (2',5'-Oligoadenylate Synthetase)
SB  - IM
MH  - 2',5'-Oligoadenylate Synthetase/genetics/*physiology
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Cells, Cultured
MH  - Genetic Vectors/genetics
MH  - *Herpesvirus 1, Human
MH  - Interferon-gamma/*genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Receptors, Interferon/genetics
MH  - Trigeminal Ganglion/cytology/immunology/*virology
MH  - eIF-2 Kinase/genetics/*physiology
MH  - Interferon gamma Receptor
PMC - PMC1472294
MID - NIHMS6004
EDAT- 2006/05/18 09:00
MHDA- 2006/06/27 09:00
PMCR- 2008/01/27
CRDT- 2006/05/18 09:00
PHST- 2006/05/18 09:00 [pubmed]
PHST- 2006/06/27 09:00 [medline]
PHST- 2006/05/18 09:00 [entrez]
PHST- 2008/01/27 00:00 [pmc-release]
AID - 10.1089/jir.2006.26.220 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2006 Apr;26(4):220-5. doi: 10.1089/jir.2006.26.220.