PMID- 16705090
OWN - NLM
STAT- MEDLINE
DCOM- 20061005
LR  - 20220331
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 108
IP  - 5
DP  - 2006 Sep 1
TI  - Integration of global SNP-based mapping and expression arrays reveals key 
      regions, mechanisms, and genes important in the pathogenesis of multiple myeloma.
PG  - 1733-43
AB  - Multiple myeloma is characterized by genomic alterations frequently involving 
      gains and losses of chromosomes. Single nucleotide polymorphism (SNP)-based 
      mapping arrays allow the identification of copy number changes at the 
      sub-megabase level and the identification of loss of heterozygosity (LOH) due to 
      monosomy and uniparental disomy (UPD). We have found that SNP-based mapping array 
      data and fluorescence in situ hybridization (FISH) copy number data correlated 
      well, making the technique robust as a tool to investigate myeloma genomics. The 
      most frequently identified alterations are located at 1p, 1q, 6q, 8p, 13, and 
      16q. LOH is found in these large regions and also in smaller regions throughout 
      the genome with a median size of 1 Mb. We have identified that UPD is prevalent 
      in myeloma and occurs through a number of mechanisms including mitotic 
      nondisjunction and mitotic recombination. For the first time in myeloma, 
      integration of mapping and expression data has allowed us to reduce the 
      complexity of standard gene expression data and identify candidate genes 
      important in both the transition from normal to monoclonal gammopathy of unknown 
      significance (MGUS) to myeloma and in different subgroups within myeloma. We have 
      documented these genes, providing a focus for further studies to identify and 
      characterize those that are key in the pathogenesis of myeloma.
FAU - Walker, Brian A
AU  - Walker BA
AD  - Section of Haemato-Oncology, Institute for Cancer Research, 15 Cotswold Rd, 
      Sutton, Surrey, United Kingdom.
FAU - Leone, Paola E
AU  - Leone PE
FAU - Jenner, Matthew W
AU  - Jenner MW
FAU - Li, Cheng
AU  - Li C
FAU - Gonzalez, David
AU  - Gonzalez D
FAU - Johnson, David C
AU  - Johnson DC
FAU - Ross, Fiona M
AU  - Ross FM
FAU - Davies, Faith E
AU  - Davies FE
FAU - Morgan, Gareth J
AU  - Morgan GJ
LA  - eng
GR  - G0100132/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060516
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Bone Marrow/pathology
MH  - *Chromosome Mapping
MH  - Gene Expression Regulation, Neoplastic
MH  - Genome, Human
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Multiple Myeloma/*genetics/pathology
MH  - Oligonucleotide Array Sequence Analysis
MH  - *Polymorphism, Single Nucleotide
MH  - Uniparental Disomy/genetics
EDAT- 2006/05/18 09:00
MHDA- 2006/10/06 09:00
CRDT- 2006/05/18 09:00
PHST- 2006/05/18 09:00 [pubmed]
PHST- 2006/10/06 09:00 [medline]
PHST- 2006/05/18 09:00 [entrez]
AID - S0006-4971(20)52647-5 [pii]
AID - 10.1182/blood-2006-02-005496 [doi]
PST - ppublish
SO  - Blood. 2006 Sep 1;108(5):1733-43. doi: 10.1182/blood-2006-02-005496. Epub 2006 
      May 16.