PMID- 16705092
OWN - NLM
STAT- MEDLINE
DCOM- 20061016
LR  - 20220408
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 108
IP  - 6
DP  - 2006 Sep 15
TI  - Transforming growth factor-beta1 regulates macrophage migration via RhoA.
PG  - 1821-9
AB  - Brief treatment with transforming growth factor (TGF)-beta1 stimulated the 
      migration of macrophages, whereas long-term exposure decreased their migration. 
      Cell migration stimulated by TGF-beta1 was markedly inhibited by 10 mug/mL Tat-C3 
      exoenzyme. TGF-beta1 increased mRNA and protein levels of macrophage inflammatory 
      protein (MIP)-1alpha in the initial period, and these effects also were inhibited 
      by 10 mug/mL Tat-C3 and a dominant-negative (DN)-RhoA (N19RhoA). Cycloheximide, 
      actinomycin D, and antibodies against MIP-1alpha and monocyte chemoattractant 
      protein-1 (MCP-1) abolished the stimulation of cell migration by TGF-beta1. These 
      findings suggest that migration of these cells is regulated directly and 
      indirectly via the expression of chemokines such as MIP-1alpha and MCP-1 mediated 
      by RhoA in response to TGF-beta1. TGF-beta1 activated RhoA in the initial period, 
      and thereafter inactivated them, suggesting that the inactivation of RhoA may be 
      the cause of the reduced cell migration in response to TGF-beta1 at later times. 
      We therefore attempted to elucidate the molecular mechanism of the inactivation 
      of RhoA by TGF-beta1. First, TGF-beta1 phosphorylated RhoA via protein kinase A, 
      leading to inactivation of RhoA. Second, wild-type p190 Rho GTPase activating 
      protein (p190RhoGAP) reduced and DN-p190RhoGAP reversed the reduction of cell 
      migration induced by TGF-beta, suggesting that it inactivated RhoA via p190 Rho 
      GAP.
FAU - Kim, Jun-Sub
AU  - Kim JS
AD  - Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, 
      Kangwon-Do 200-702, Korea.
FAU - Kim, Jae-Gyu
AU  - Kim JG
FAU - Moon, Mi-Young
AU  - Moon MY
FAU - Jeon, Chan-Young
AU  - Jeon CY
FAU - Won, Ha-Young
AU  - Won HY
FAU - Kim, Hee-Jun
AU  - Kim HJ
FAU - Jeon, Yee-Jin
AU  - Jeon YJ
FAU - Seo, Ji-Yeon
AU  - Seo JY
FAU - Kim, Jong-Il
AU  - Kim JI
FAU - Kim, Jaebong
AU  - Kim J
FAU - Lee, Jae-Yong
AU  - Lee JY
FAU - Kim, Pyeung-Hyeun
AU  - Kim PH
FAU - Park, Jae-Bong
AU  - Park JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060516
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (ARHGAP35 protein, human)
RN  - 0 (ARHGAP5 protein, human)
RN  - 0 (Arhgap35 protein, mouse)
RN  - 0 (Arhgap5 protein, mouse)
RN  - 0 (Carrier Proteins)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/genetics/metabolism
MH  - Cell Line
MH  - Cell Movement/drug effects/physiology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemotaxis/drug effects/physiology
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - DNA-Binding Proteins
MH  - GTPase-Activating Proteins
MH  - Gene Expression/drug effects
MH  - Guanine Nucleotide Exchange Factors
MH  - HL-60 Cells
MH  - Humans
MH  - Macrophage Activation/drug effects/physiology
MH  - Macrophage Inflammatory Proteins/genetics/metabolism
MH  - Macrophages/*drug effects/*physiology
MH  - Mice
MH  - Models, Biological
MH  - RNA, Messenger/genetics/metabolism
MH  - Repressor Proteins
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Transforming Growth Factor beta1
MH  - rho GTP-Binding Proteins/metabolism
MH  - rhoA GTP-Binding Protein/*antagonists & inhibitors/physiology
EDAT- 2006/05/18 09:00
MHDA- 2006/10/17 09:00
CRDT- 2006/05/18 09:00
PHST- 2006/05/18 09:00 [pubmed]
PHST- 2006/10/17 09:00 [medline]
PHST- 2006/05/18 09:00 [entrez]
AID - S0006-4971(20)52552-4 [pii]
AID - 10.1182/blood-2005-10-009191 [doi]
PST - ppublish
SO  - Blood. 2006 Sep 15;108(6):1821-9. doi: 10.1182/blood-2005-10-009191. Epub 2006 
      May 16.